Generation and grafting of tissue-engineered vessels in a mouse model

Mei Mei Wong, Xuechong Hong, Eirini Karamariti, Yanhua Hu, Qingbo Xu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. The current protocol presents an efficient and reproducible strategy in which functional tissue engineered vessel grafts can be generated using partially induced pluripotent stem cell (PiPSC) from human fibroblasts. We designed a decellularized vessel scaffold bioreactor, which closely mimics the matrix protein structure and blood flow that exists within a native vessel, for seeding of PiPSC-endothelial cells or smooth muscle cells prior to grafting into mice. This approach was demonstrated to be advantageous because immune-deficient mice engrafted with the PiPSC-derived grafts presented with markedly increased survival rate 3 weeks after surgery. This protocol represents a valuable tool for regenerative medicine, tissue engineering and potentially patient-specific cell-therapy in the near future.

Original languageEnglish
Article numbere52565
JournalJournal of Visualized Experiments
Issue number97
Publication statusPublished - 18 Mar 2015


  • Bioengineering
  • Bioreactor
  • Issue 97
  • Mouse models
  • Partially induced pluripotent stem cells
  • Stem cells
  • Tissue engineering
  • Vascular differentiation
  • Vessel graft


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