Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulation

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
47 Downloads (Pure)

Abstract

Dual co-stimulation may be harnessed using parallel chimeric antigen receptors
(pCARs) in which two distinct co-stimulatory units are adjacently localized on
the plasma membrane. This protocol summarizes construct design, human T
cell isolation, retroviral transduction, tissue culture expansion, and preclinical
testing of pCAR T cells, exemplified by receptors that co-target avb6 integrin
and ErbB dimers.
For complete details on the use and execution of this protocol, please refer to
Muliaditan et al. (2021).
Original languageEnglish
Article number101414
Number of pages28
JournalSTAR Protocols
Volume3
Issue number2
DOIs
Publication statusPublished - 17 Jun 2022

Fingerprint

Dive into the research topics of 'Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulation'. Together they form a unique fingerprint.

Cite this