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Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene

Research output: Contribution to journalArticle

Cristian Miere, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)37-39
Number of pages3
JournalStem Cell Research
Volume16
Issue number1
Early online date3 Dec 2015
DOIs
Accepted/In press2 Dec 2015
E-pub ahead of print3 Dec 2015
PublishedJan 2016

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Abstract

The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3A > T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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