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Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

Research output: Contribution to journalArticle

Cristian Miere, Heema Hewitson, Liani Devito, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)342-344
Number of pages3
JournalStem Cell Research
Volume16
Issue number2
Early online date15 Jan 2016
DOIs
Publication statusPublished - Mar 2016

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Abstract

The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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