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Generation of KCL021 research grade human embryonic stem cell line carrying a δF508 mutation in the CFTR gene

Research output: Contribution to journalArticle

Cristian Miere, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)177-179
Number of pages3
JournalStem Cell Research
Volume16
Issue number1
Early online date6 Jan 2016
DOIs
Accepted/In press31 Dec 2015
E-pub ahead of print6 Jan 2016
PublishedJan 2016

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Abstract

The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a δF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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