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Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

Research output: Contribution to journalArticle

Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)256-258
Number of pages3
JournalStem Cell Research
Volume16
Issue number2
Early online date14 Jan 2016
DOIs
Accepted/In press12 Jan 2016
E-pub ahead of print14 Jan 2016
PublishedMar 2016

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Abstract

The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739-3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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