Generation of KCL027 research grade human embryonic stem cell line carrying a mutation in the HTT gene

Laureen Jacquet, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Carl Hobbs, Emma Stephenson, Dusko Ilic*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

85 Downloads (Pure)

Abstract

The KCL027 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

Original languageEnglish
Pages (from-to)274-277
Number of pages4
JournalStem Cell Research
Volume16
Issue number2
Early online date14 Jan 2016
DOIs
Publication statusPublished - Mar 2016

Fingerprint

Dive into the research topics of 'Generation of KCL027 research grade human embryonic stem cell line carrying a mutation in the HTT gene'. Together they form a unique fingerprint.

Cite this