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Generation of KCL027 research grade human embryonic stem cell line carrying a mutation in the HTT gene

Research output: Contribution to journalArticle

Laureen Jacquet, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Carl Hobbs, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)274-277
Number of pages4
JournalStem Cell Research
Volume16
Issue number2
Early online date14 Jan 2016
DOIs
Accepted/In press12 Jan 2016
E-pub ahead of print14 Jan 2016
PublishedMar 2016

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Abstract

The KCL027 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

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