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Generation of KCL031 clinical grade human embryonic stem cell line

Research output: Contribution to journalArticle

Laureen Jacquet, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Carl Hobbs, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalStem Cell Research
Issue number1
Early online date3 Jan 2016
Accepted/In press29 Dec 2015
E-pub ahead of print3 Jan 2016
PublishedJan 2016


King's Authors


The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

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