Generation of KCL033 clinical grade human embryonic stem cell line

Liani Devito; Anastasia Petrova; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.047

Generation of KCL033 clinical grade human embryonic stem cell line

Liani Devito, Anastasia Petrova, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

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Abstract

The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.

Original language	English
Pages (from-to)	296-299
Number of pages	4
Journal	Stem Cell Research
Volume	16
Issue number	2
Early online date	15 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2015.12.047
Publication status	Published - Mar 2016

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Generation of KCL033 clinical_DEVITO_Publishedonline15January2016_GOLD VoR (CC BY)Final published version, 495 KBLicence: CC BY

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abstract = "The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.",

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AU - Devito, Liani

AU - Petrova, Anastasia

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

PY - 2016/3

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AB - The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.

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Generation of KCL033 clinical grade human embryonic stem cell line

Abstract

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