Research output: Contribution to journal › Article
Liani Devito, Anastasia Petrova, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
| Original language | English |
|---|---|
| Pages (from-to) | 296-299 |
| Number of pages | 4 |
| Journal | Stem Cell Research |
| Volume | 16 |
| Issue number | 2 |
| Early online date | 15 Jan 2016 |
| DOIs | |
| Publication status | Published - Mar 2016 |
Generation of KCL033 clinical_DEVITO_Publishedonline15January2016_GOLD VoR (CC BY)
Generation_of_KCL033_clinical_DEVITO_Publishedonline15January2016_GOLD_VoR_CC_BY_.pdf, 495 KB, application/pdf
2/08/2017
Final published version
CC BY
The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.
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