Generation of KCL034 clinical grade human embryonic stem cell line

Liani Devito; Laureen Jacquet; Anastasia Petrova; Cristian Miere; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.034

Generation of KCL034 clinical grade human embryonic stem cell line

Liani Devito, Laureen Jacquet, Anastasia Petrova, Cristian Miere, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

109 Downloads (Pure)

Abstract

The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility, specific and non-specific human pathogens.

Original language	English
Pages (from-to)	184-188
Number of pages	5
Journal	Stem Cell Research
Volume	16
Issue number	1
Early online date	3 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2015.12.034
Publication status	Published - Jan 2016

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Generation of KCL034 clinical_DEVITO_Publishedonline3January2016_GOLD VoR (CC BY)Final published version, 1.17 MBLicence: CC BY

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author = "Liani Devito and Laureen Jacquet and Anastasia Petrova and Cristian Miere and Victoria Wood and Neli Kadeva and Glenda Cornwell and Stefano Codognotto and Emma Stephenson and Dusko Ilic",

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AU - Devito, Liani

AU - Jacquet, Laureen

AU - Petrova, Anastasia

AU - Miere, Cristian

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

PY - 2016/1

Y1 - 2016/1

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AB - The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility, specific and non-specific human pathogens.

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JO - Stem Cell Research

JF - Stem Cell Research

IS - 1

ER -

Generation of KCL034 clinical grade human embryonic stem cell line

Abstract

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