Research output: Contribution to journal › Article › peer-review
Liani Devito, Laureen Jacquet, Anastasia Petrova, Cristian Miere, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
| Original language | English |
|---|---|
| Pages (from-to) | 184-188 |
| Number of pages | 5 |
| Journal | Stem Cell Research |
| Volume | 16 |
| Issue number | 1 |
| Early online date | 3 Jan 2016 |
| DOIs | |
| Accepted/In press | 29 Dec 2015 |
| E-pub ahead of print | 3 Jan 2016 |
| Published | Jan 2016 |
| Additional links |
Generation of KCL034 clinical_DEVITO_Publishedonline3January2016_GOLD VoR (CC BY)
Generation_of_KCL034_clinical_DEVITO_Publishedonline3January2016_GOLD_VoR_CC_BY_.pdf, 1.17 MB, application/pdf
Uploaded date:02 Aug 2017
Version:Final published version
Licence:CC BY
The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility, specific and non-specific human pathogens.
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