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Generation of KCL034 clinical grade human embryonic stem cell line

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)184-188
Number of pages5
JournalStem Cell Research
Volume16
Issue number1
Early online date3 Jan 2016
DOIs
Publication statusPublished - Jan 2016

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Abstract

The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility, specific and non-specific human pathogens.

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