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Generation of KCL037 clinical grade human embryonic stem cell line

Research output: Contribution to journalArticle

Cristian Miere, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic

Original languageEnglish
Pages (from-to)149-151
Number of pages3
JournalStem Cell Research
Volume16
Issue number1
Early online date29 Dec 2015
DOIs
Accepted/In press25 Dec 2015
E-pub ahead of print29 Dec 2015
PublishedJan 2016

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Abstract

The KCL037 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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