TY - JOUR
T1 - Genetic regulation of mouse glycosylphosphatidylinositol-phospholipase D
AU - Flores-Borja, F
AU - Kieszkievicz, J
AU - Church, V
AU - Francis-West, P H
AU - Schofield, J
AU - Rademacher, T W
AU - Lund, T
PY - 2004/4
Y1 - 2004/4
N2 - Glycosylphosphatidylinositol phospholipase D (GPI-PLD) has been proposed to be responsible for cleaving membrane-associated glycosylphosphatidyl inositol (GPI) molecules to generate inositol phosphoglycan (IPGs), which have growth factor-mimetic properties. We have cloned the mouse liver GPI-PLD cDNA, which has a sequence that differs from that previously isolated from a mouse glucagonoma cell library. Using a highly specific and very sensitive RNase protection assay, we found that the GPI-PLD expressed in adult/post-natal brain, antrum and insulin-producing cells is identical to that isolated from liver. The expression of mouse GPI-PLD in liver shows a complex genetic regulation with a mouse strain-specific variation. In addition, GPI-PLD mRNA levels were higher in 4-week old animals compared to older animals, and the GPI-PLD mRNA levels increased in mice that developed insulin dependent type 1 diabetes spontaneously. This suggests that the expression of liver GPI-PLD in mice is highly regulated. (C) 2004 Elsevier SAS. All rights reserved.
AB - Glycosylphosphatidylinositol phospholipase D (GPI-PLD) has been proposed to be responsible for cleaving membrane-associated glycosylphosphatidyl inositol (GPI) molecules to generate inositol phosphoglycan (IPGs), which have growth factor-mimetic properties. We have cloned the mouse liver GPI-PLD cDNA, which has a sequence that differs from that previously isolated from a mouse glucagonoma cell library. Using a highly specific and very sensitive RNase protection assay, we found that the GPI-PLD expressed in adult/post-natal brain, antrum and insulin-producing cells is identical to that isolated from liver. The expression of mouse GPI-PLD in liver shows a complex genetic regulation with a mouse strain-specific variation. In addition, GPI-PLD mRNA levels were higher in 4-week old animals compared to older animals, and the GPI-PLD mRNA levels increased in mice that developed insulin dependent type 1 diabetes spontaneously. This suggests that the expression of liver GPI-PLD in mice is highly regulated. (C) 2004 Elsevier SAS. All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=2942631202&partnerID=8YFLogxK
U2 - 10.1016/j.biochi.2004.04.003
DO - 10.1016/j.biochi.2004.04.003
M3 - Article
VL - 86
SP - 275
EP - 282
JO - Biochimie
JF - Biochimie
IS - 4-5
ER -
