Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

Michaela Frye; Ilaria Dragoni; Suet-Feung Chin; Immaculada Spiteri; Agata Kurowski; Elena Provenzano; Andrew Green; Ian O Ellis; Donna Grimmer; Andrew Teschendorff; Christos C Zouboulis; Carlos Caldas; Fiona M Watt

doi:10.1016/j.canlet.2009.08.004

Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

Michaela Frye, Ilaria Dragoni, Suet-Feung Chin, Immaculada Spiteri, Agata Kurowski, Elena Provenzano, Andrew Green, Ian O Ellis, Donna Grimmer, Andrew Teschendorff, Christos C Zouboulis, Carlos Caldas, Fiona M Watt

Gene Therapy and Regenerative Medicine

University of Cambridge

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.

Original language	English
Article number	N/A
Pages (from-to)	71-80
Number of pages	10
Journal	Cancer Letters
Volume	289
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2009.08.004
Publication status	Published - 1 Mar 2010

Keywords

Blotting, Western
Breast Neoplasms
Cell Survival
Chromosomes, Human, Pair 5
Female
Gene Dosage
Gene Expression Profiling
Genetic Predisposition to Disease
Genome, Human
Humans
Methyltransferases
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2009.08.004

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title = "Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer",

abstract = "We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.",

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author = "Michaela Frye and Ilaria Dragoni and Suet-Feung Chin and Immaculada Spiteri and Agata Kurowski and Elena Provenzano and Andrew Green and Ellis, {Ian O} and Donna Grimmer and Andrew Teschendorff and Zouboulis, {Christos C} and Carlos Caldas and Watt, {Fiona M}",

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doi = "10.1016/j.canlet.2009.08.004",

language = "English",

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journal = "Cancer Letters",

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T1 - Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

AU - Frye, Michaela

AU - Dragoni, Ilaria

AU - Chin, Suet-Feung

AU - Spiteri, Immaculada

AU - Kurowski, Agata

AU - Provenzano, Elena

AU - Green, Andrew

AU - Ellis, Ian O

AU - Grimmer, Donna

AU - Teschendorff, Andrew

AU - Zouboulis, Christos C

AU - Caldas, Carlos

AU - Watt, Fiona M

PY - 2010/3/1

Y1 - 2010/3/1

N2 - We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.

AB - We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.

KW - Blotting, Western

KW - Breast Neoplasms

KW - Cell Survival

KW - Chromosomes, Human, Pair 5

KW - Female

KW - Gene Dosage

KW - Gene Expression Profiling

KW - Genetic Predisposition to Disease

KW - Genome, Human

KW - Humans

KW - Methyltransferases

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Up-Regulation

U2 - 10.1016/j.canlet.2009.08.004

DO - 10.1016/j.canlet.2009.08.004

M3 - Article

C2 - 19740597

SN - 0304-3835

VL - 289

SP - 71

EP - 80

JO - Cancer Letters

JF - Cancer Letters

IS - 1

M1 - N/A

ER -

Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

Abstract

Keywords

UN SDGs

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