GluNs Detection and Functions in Microglial Cells

Stéphane Peineau; Vincent Degos; Catherine Verney; Pierre Gressens

doi:10.1007/978-1-4939-7321-7_14

GluNs Detection and Functions in Microglial Cells

Stéphane Peineau, Vincent Degos, Catherine Verney, Pierre Gressens

Centre for the Developing Brain

Research output: Contribution to journal › Article › peer-review

Abstract

Proving endogenous GluN presence and functions in microglia require complementary steps to demonstrate (1) that GluN genes are transcripted and translated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The complete demonstration is performed by using mRNA detection technics, western blots, immunofluorescence, electrophysiology, calcium imaging, morphology studies, multiplex immunoassay together with conditional microglial Knock-Out mice and brain lesion models.

Original language	English
Pages (from-to)	253-263
Number of pages	11
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1677
DOIs	https://doi.org/10.1007/978-1-4939-7321-7_14
Publication status	Published - 2017

Keywords

Journal Article

Access to Document

10.1007/978-1-4939-7321-7_14

Cite this

@article{ed8657a60e8d4893b7db38d35c6a2319,

title = "GluNs Detection and Functions in Microglial Cells",

abstract = "Proving endogenous GluN presence and functions in microglia require complementary steps to demonstrate (1) that GluN genes are transcripted and translated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The complete demonstration is performed by using mRNA detection technics, western blots, immunofluorescence, electrophysiology, calcium imaging, morphology studies, multiplex immunoassay together with conditional microglial Knock-Out mice and brain lesion models.",

keywords = "Journal Article",

author = "St{\'e}phane Peineau and Vincent Degos and Catherine Verney and Pierre Gressens",

year = "2017",

doi = "10.1007/978-1-4939-7321-7_14",

language = "English",

volume = "1677",

pages = "253--263",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1940-6029",

}

TY - JOUR

T1 - GluNs Detection and Functions in Microglial Cells

AU - Peineau, Stéphane

AU - Degos, Vincent

AU - Verney, Catherine

AU - Gressens, Pierre

PY - 2017

Y1 - 2017

N2 - Proving endogenous GluN presence and functions in microglia require complementary steps to demonstrate (1) that GluN genes are transcripted and translated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The complete demonstration is performed by using mRNA detection technics, western blots, immunofluorescence, electrophysiology, calcium imaging, morphology studies, multiplex immunoassay together with conditional microglial Knock-Out mice and brain lesion models.

AB - Proving endogenous GluN presence and functions in microglia require complementary steps to demonstrate (1) that GluN genes are transcripted and translated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The complete demonstration is performed by using mRNA detection technics, western blots, immunofluorescence, electrophysiology, calcium imaging, morphology studies, multiplex immunoassay together with conditional microglial Knock-Out mice and brain lesion models.

KW - Journal Article

U2 - 10.1007/978-1-4939-7321-7_14

DO - 10.1007/978-1-4939-7321-7_14

M3 - Article

C2 - 28986878

SN - 1940-6029

VL - 1677

SP - 253

EP - 263

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

GluNs Detection and Functions in Microglial Cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this