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Granulocyte-macrophage colony stimulating factor as an indirect mediator of nociceptor activation and pain: Nociceptive effects of GM-CSF

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Damini Tewari, Andrew D. Cook, Ming-Chin Lee, Anne D. Christensen, Andrew Croxford, Burkhard Becher, Daniel Poole, Pradeep Rajasekhar, Nigel Bunnett, Julia E. Smith, John A. Hamilton, Stephen B. McMahon

Original languageEnglish
Article number2268-19
Pages (from-to)2189-2199
Number of pages11
JournalJournal of Neuroscience
Issue number10
Early online date4 Feb 2020
Publication statusPublished - 11 Mar 2020


King's Authors


The interaction between the immune system and the nervous system has been at the center of multiple research studies in recent years. Whereas the role played by cytokines as neuronal mediators is no longer contested, the mechanisms by which cytokines modulate pain processing remain to be elucidated. In this study, we have analyzed the involvement of granulocyte-macrophage colony stimulating factor (GM-CSF) in nociceptor activation in male and female mice. Previous studies have suggested GM-CSF might directly activate neurons. However, here we established the absence of a functional GM-CSF receptor in murine nociceptors, and suggest an indirect mechanism of action, via immune cells. We report that GM-CSF applied directly to magnetically purified nociceptors does not induce any transcriptional changes in nociceptive genes. In contrast, conditioned medium from GM-CSF-treated murine macrophages was able to drive nociceptor transcription. We also found that conditioned medium from nociceptors treated with the well established pain mediator, nerve growth factor, could also modify macrophage gene transcription, providing further evidence for a bidirectional crosstalk.

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