Group III metabotropic glutamate receptors act as hetero-receptors modulating evoked GABA release in the globus pallidus in vivo

N MacInnes, S Duty

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

In vitro studies suggest that group III metabotropic glutamate (mGlu) receptors function as hetero-receptors to modulate GABA release within the globus pallidus. In the present study we examined this hypothesis in vivo, using microdialysis to assess the ability of locally infused group III mGlu receptor agonists to modulate KCl-evoked GABA release in the globus pallidus of anaesthetised rats. Extra-cellular levels of GABA in dialysate samples were assayed using High Pressure Liquid Chromatography (HPLC) coupled to electrochemical detection. Infusion of KCl (50100 mM) evoked a dose-dependent increase in GABA levels in the globus pallidus. Addition of the group III mGlu receptor agonists L-AP4 (30 and 300 mu M) and L-SOP (3-300 mu M) significantly reduced the extra-synaptic levels of GABA that resulted after 100 mM KCl challenge. The effect Of L-SOP (30 mu M) was almost totally abolished by co-infusion with M-SOP (30 mu M). These data confirm that activation of group III mGlu receptors inhibits GABA release in the globus pallidus, thereby supporting their hetero-receptor role in vivo. (C) 2007 Elsevier B.V. All rights reserved
Original languageEnglish
Pages (from-to)95 - 99
Number of pages5
JournalEuropean Journal of Pharmacology
Volume580
Issue number1-2
DOIs
Publication statusPublished - 2 Feb 2008

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