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Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos

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Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos. / Gressens, P; Hill, J M; Gozes, I; Fridkin, M; Brenneman, D E; Gressens, Pierre.

In: NATURE, Vol. 362, No. 6416, 11.03.1993, p. 155-8.

Research output: Contribution to journalArticle

Harvard

Gressens, P, Hill, JM, Gozes, I, Fridkin, M, Brenneman, DE & Gressens, P 1993, 'Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos', NATURE, vol. 362, no. 6416, pp. 155-8. https://doi.org/10.1038/362155a0

APA

Gressens, P., Hill, J. M., Gozes, I., Fridkin, M., Brenneman, D. E., & Gressens, P. (1993). Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos. NATURE, 362(6416), 155-8. https://doi.org/10.1038/362155a0

Vancouver

Gressens P, Hill JM, Gozes I, Fridkin M, Brenneman DE, Gressens P. Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos. NATURE. 1993 Mar 11;362(6416):155-8. https://doi.org/10.1038/362155a0

Author

Gressens, P ; Hill, J M ; Gozes, I ; Fridkin, M ; Brenneman, D E ; Gressens, Pierre. / Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos. In: NATURE. 1993 ; Vol. 362, No. 6416. pp. 155-8.

Bibtex Download

@article{cdc89063a6b742099b5009f641940317,
title = "Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos",
abstract = "Factors controlling central nervous system (CNS) growth immediately after neurulation are mostly unknown. Vasoactive intestinal peptide (VIP) receptors are widely distributed in the embryonic nervous system, and VIP has trophic and mitogenic properties on embryonic neural tissues but inhibits growth and mitosis in certain tumours. To address the potential effects of VIP on embryonic growth, we used whole postimplantation embryo cultures. After a 4-h incubation, VIP stimulated growth, increasing somite number, embryonic volume, DNA and protein content, and number of cells in S-phase. A VIP antagonist substantially inhibited these VIP-mediated increments in growth. The VIP antagonist completely suppressed VIP-stimulated mitosis in the CNS while decreasing the same in non-neuronal tissues by 38%. In vitro autoradiography revealed GTP-sensitive and GTP-insensitive VIP receptors which were differentially regulated in VIP antagonist-treated embryos. The present study suggests that VIP acts as a growth factor on early postimplantation embryos through multiple VIP receptors that exhibit tissue-specific responses.",
keywords = "Analysis of Variance, Animals, Cell Division, Central Nervous System, DNA, Embryo, Mammalian, GTP-Binding Proteins, Growth Substances, Mice, Organ Culture Techniques, Proteins, Receptors, Gastrointestinal Hormone, Receptors, Vasoactive Intestinal Peptide, S Phase, Secretin, Vasoactive Intestinal Peptide",
author = "P Gressens and Hill, {J M} and I Gozes and M Fridkin and Brenneman, {D E} and Pierre Gressens",
year = "1993",
month = mar,
day = "11",
doi = "10.1038/362155a0",
language = "English",
volume = "362",
pages = "155--8",
journal = "NATURE",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6416",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos

AU - Gressens, P

AU - Hill, J M

AU - Gozes, I

AU - Fridkin, M

AU - Brenneman, D E

AU - Gressens, Pierre

PY - 1993/3/11

Y1 - 1993/3/11

N2 - Factors controlling central nervous system (CNS) growth immediately after neurulation are mostly unknown. Vasoactive intestinal peptide (VIP) receptors are widely distributed in the embryonic nervous system, and VIP has trophic and mitogenic properties on embryonic neural tissues but inhibits growth and mitosis in certain tumours. To address the potential effects of VIP on embryonic growth, we used whole postimplantation embryo cultures. After a 4-h incubation, VIP stimulated growth, increasing somite number, embryonic volume, DNA and protein content, and number of cells in S-phase. A VIP antagonist substantially inhibited these VIP-mediated increments in growth. The VIP antagonist completely suppressed VIP-stimulated mitosis in the CNS while decreasing the same in non-neuronal tissues by 38%. In vitro autoradiography revealed GTP-sensitive and GTP-insensitive VIP receptors which were differentially regulated in VIP antagonist-treated embryos. The present study suggests that VIP acts as a growth factor on early postimplantation embryos through multiple VIP receptors that exhibit tissue-specific responses.

AB - Factors controlling central nervous system (CNS) growth immediately after neurulation are mostly unknown. Vasoactive intestinal peptide (VIP) receptors are widely distributed in the embryonic nervous system, and VIP has trophic and mitogenic properties on embryonic neural tissues but inhibits growth and mitosis in certain tumours. To address the potential effects of VIP on embryonic growth, we used whole postimplantation embryo cultures. After a 4-h incubation, VIP stimulated growth, increasing somite number, embryonic volume, DNA and protein content, and number of cells in S-phase. A VIP antagonist substantially inhibited these VIP-mediated increments in growth. The VIP antagonist completely suppressed VIP-stimulated mitosis in the CNS while decreasing the same in non-neuronal tissues by 38%. In vitro autoradiography revealed GTP-sensitive and GTP-insensitive VIP receptors which were differentially regulated in VIP antagonist-treated embryos. The present study suggests that VIP acts as a growth factor on early postimplantation embryos through multiple VIP receptors that exhibit tissue-specific responses.

KW - Analysis of Variance

KW - Animals

KW - Cell Division

KW - Central Nervous System

KW - DNA

KW - Embryo, Mammalian

KW - GTP-Binding Proteins

KW - Growth Substances

KW - Mice

KW - Organ Culture Techniques

KW - Proteins

KW - Receptors, Gastrointestinal Hormone

KW - Receptors, Vasoactive Intestinal Peptide

KW - S Phase

KW - Secretin

KW - Vasoactive Intestinal Peptide

U2 - 10.1038/362155a0

DO - 10.1038/362155a0

M3 - Article

C2 - 8383805

VL - 362

SP - 155

EP - 158

JO - NATURE

JF - NATURE

SN - 0028-0836

IS - 6416

ER -

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