Haemophilus influenzae as a possible cause of Guillain-Barre syndrome

Y Y Ju; H Womersley; J Pritchard; I Gray; R A C Hughes; N A Gregson

doi:10.1016/j.jneuroim.2003.12.011

Haemophilus influenzae as a possible cause of Guillain-Barre syndrome

Y Y Ju, H Womersley, J Pritchard, I Gray, R A C Hughes, N A Gregson

King's College London

Research output: Contribution to journal › Article › peer-review

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Abstract

Recent reports have contained conflicting results on the relationship between antecedent Haemophilus influenzae infection and Guillain-Barre syndrome (GBS). To investigate the prevalence of H. influenzae infection in GBS patients in a British population, we carried out a retrospective study with 62 consecutive GBS patients and 63 normal controls of similar age and sex. Whole bacteria of both encapsulated and nonencapsulated strains of H. influenzae were employed as antigens in an enzyme-linked immunosorbent assay (ELISA) for anti-H. influenzae IgG, IgM and IgA antibodies. Elevated antibodies of two or three classes were found in one GBS patient and none in the normal controls. Six GBS patients had IgG antibodies against nonencapsulated H. influenzae compared with only one in the normal control.group (p=0.06). Western blot for IgG antibody showed that all the sera with IgG antibodies recognized the lipopolysaccharide (LPS) of both strains of H. influenzae. Antiganglioside GMI antibody was not associated with anti-H. influenzae antibody in our study. Absorption with encapsulated or nonencapsulated H. influenzae, Campylobacter jejuni and Escherichia coli before testing on Western blot showed that only nonencapsulated H. influenzae absorbed the anti-LPS antibodies. In conclusion, there is a possible but rare association of GBS with nonencapsulated H. influenzae in the UK. (C) 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	160 - 166
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	149
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2003.12.011
Publication status	Published - Apr 2004

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10.1016/j.jneuroim.2003.12.011

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title = "Haemophilus influenzae as a possible cause of Guillain-Barre syndrome",

abstract = "Recent reports have contained conflicting results on the relationship between antecedent Haemophilus influenzae infection and Guillain-Barre syndrome (GBS). To investigate the prevalence of H. influenzae infection in GBS patients in a British population, we carried out a retrospective study with 62 consecutive GBS patients and 63 normal controls of similar age and sex. Whole bacteria of both encapsulated and nonencapsulated strains of H. influenzae were employed as antigens in an enzyme-linked immunosorbent assay (ELISA) for anti-H. influenzae IgG, IgM and IgA antibodies. Elevated antibodies of two or three classes were found in one GBS patient and none in the normal controls. Six GBS patients had IgG antibodies against nonencapsulated H. influenzae compared with only one in the normal control.group (p=0.06). Western blot for IgG antibody showed that all the sera with IgG antibodies recognized the lipopolysaccharide (LPS) of both strains of H. influenzae. Antiganglioside GMI antibody was not associated with anti-H. influenzae antibody in our study. Absorption with encapsulated or nonencapsulated H. influenzae, Campylobacter jejuni and Escherichia coli before testing on Western blot showed that only nonencapsulated H. influenzae absorbed the anti-LPS antibodies. In conclusion, there is a possible but rare association of GBS with nonencapsulated H. influenzae in the UK. (C) 2004 Elsevier B.V. All rights reserved.",

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Haemophilus influenzae as a possible cause of Guillain-Barre syndrome

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