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HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication

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HFE mRNA expression is responsive to intracellular and extracellular iron loading : short communication. / Mehta, Kosha J.; Farnaud, Sebastien; Patel, Vinood B.

In: Molecular Biology Reports, Vol. 44, No. 5, 10.2017, p. 399-403.

Research output: Contribution to journalArticlepeer-review

Harvard

Mehta, KJ, Farnaud, S & Patel, VB 2017, 'HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication', Molecular Biology Reports, vol. 44, no. 5, pp. 399-403. https://doi.org/10.1007/s11033-017-4123-2

APA

Mehta, K. J., Farnaud, S., & Patel, V. B. (2017). HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication. Molecular Biology Reports, 44(5), 399-403. https://doi.org/10.1007/s11033-017-4123-2

Vancouver

Mehta KJ, Farnaud S, Patel VB. HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication. Molecular Biology Reports. 2017 Oct;44(5):399-403. https://doi.org/10.1007/s11033-017-4123-2

Author

Mehta, Kosha J. ; Farnaud, Sebastien ; Patel, Vinood B. / HFE mRNA expression is responsive to intracellular and extracellular iron loading : short communication. In: Molecular Biology Reports. 2017 ; Vol. 44, No. 5. pp. 399-403.

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@article{50ba6c24773640bea5c627948da77d88,
title = "HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication",
abstract = "In liver hepatocytes, the HFE gene regulates cellular and systemic iron homeostasis by modulating cellular iron-uptake and producing the iron-hormone hepcidin in response to systemic iron elevation. However, the mechanism of iron-sensing in hepatocytes remain enigmatic. Therefore, to study the effect of iron on HFE and hepcidin (HAMP) expressions under distinct extracellular and intracellular iron-loading, we examined the effect of holotransferrin treatment (1, 2, 5 and 8 g/L for 6 h) on intracellular iron levels, and mRNA expressions of HFE and HAMP in wild-type HepG2 and previously characterized iron-loaded recombinant-TfR1 HepG2 cells. Gene expression was analyzed by real-time PCR and intracellular iron was measured by ferrozine assay. Data showed that in the wild-type cells, where intracellular iron content remained unchanged, HFE expression remained unaltered at low holotransferrin treatments but was upregulated upon 5 g/L (p < 0.04) and 8 g/L (p = 0.05) treatments. HAMP expression showed alternating elevations and increased upon 1 g/L (p < 0.05) and 5 g/L (p < 0.05). However, in the recombinant cells that showed higher intracellular iron levels than wild-type cells, HFE and HAMP expressions were elevated only at low 1 g/L treatment (p < 0.03) and were repressed at 2 g/L treatment (p < 0.03). Under holotransferrin-untreated conditions, the iron-loaded recombinant cells showed higher expressions of HFE (p < 0.03) and HAMP (p = 0.05) than wild-type cells. HFE mRNA was independently elevated by extracellular and intracellular iron-excess. Thus, it may be involved in sensing both, extracellular and intracellular iron. Repression of HAMP expression under simultaneous intracellular and extracellular iron-loading resembles non-hereditary iron-excess pathologies.",
keywords = "Antigens, CD/metabolism, Cells, Cultured, Gene Expression Regulation, Hemochromatosis/genetics, Hemochromatosis Protein/genetics, Hep G2 Cells, Hepatocytes/metabolism, Hepcidins/genetics, Histocompatibility Antigens Class I/genetics, Homeostasis, Humans, Iron/metabolism, Liver/metabolism, RNA, Messenger/genetics, Real-Time Polymerase Chain Reaction, Receptors, Transferrin/metabolism, Transferrin/metabolism",
author = "Mehta, {Kosha J.} and Sebastien Farnaud and Patel, {Vinood B.}",
year = "2017",
month = oct,
doi = "10.1007/s11033-017-4123-2",
language = "English",
volume = "44",
pages = "399--403",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "5",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - HFE mRNA expression is responsive to intracellular and extracellular iron loading

T2 - short communication

AU - Mehta, Kosha J.

AU - Farnaud, Sebastien

AU - Patel, Vinood B.

PY - 2017/10

Y1 - 2017/10

N2 - In liver hepatocytes, the HFE gene regulates cellular and systemic iron homeostasis by modulating cellular iron-uptake and producing the iron-hormone hepcidin in response to systemic iron elevation. However, the mechanism of iron-sensing in hepatocytes remain enigmatic. Therefore, to study the effect of iron on HFE and hepcidin (HAMP) expressions under distinct extracellular and intracellular iron-loading, we examined the effect of holotransferrin treatment (1, 2, 5 and 8 g/L for 6 h) on intracellular iron levels, and mRNA expressions of HFE and HAMP in wild-type HepG2 and previously characterized iron-loaded recombinant-TfR1 HepG2 cells. Gene expression was analyzed by real-time PCR and intracellular iron was measured by ferrozine assay. Data showed that in the wild-type cells, where intracellular iron content remained unchanged, HFE expression remained unaltered at low holotransferrin treatments but was upregulated upon 5 g/L (p < 0.04) and 8 g/L (p = 0.05) treatments. HAMP expression showed alternating elevations and increased upon 1 g/L (p < 0.05) and 5 g/L (p < 0.05). However, in the recombinant cells that showed higher intracellular iron levels than wild-type cells, HFE and HAMP expressions were elevated only at low 1 g/L treatment (p < 0.03) and were repressed at 2 g/L treatment (p < 0.03). Under holotransferrin-untreated conditions, the iron-loaded recombinant cells showed higher expressions of HFE (p < 0.03) and HAMP (p = 0.05) than wild-type cells. HFE mRNA was independently elevated by extracellular and intracellular iron-excess. Thus, it may be involved in sensing both, extracellular and intracellular iron. Repression of HAMP expression under simultaneous intracellular and extracellular iron-loading resembles non-hereditary iron-excess pathologies.

AB - In liver hepatocytes, the HFE gene regulates cellular and systemic iron homeostasis by modulating cellular iron-uptake and producing the iron-hormone hepcidin in response to systemic iron elevation. However, the mechanism of iron-sensing in hepatocytes remain enigmatic. Therefore, to study the effect of iron on HFE and hepcidin (HAMP) expressions under distinct extracellular and intracellular iron-loading, we examined the effect of holotransferrin treatment (1, 2, 5 and 8 g/L for 6 h) on intracellular iron levels, and mRNA expressions of HFE and HAMP in wild-type HepG2 and previously characterized iron-loaded recombinant-TfR1 HepG2 cells. Gene expression was analyzed by real-time PCR and intracellular iron was measured by ferrozine assay. Data showed that in the wild-type cells, where intracellular iron content remained unchanged, HFE expression remained unaltered at low holotransferrin treatments but was upregulated upon 5 g/L (p < 0.04) and 8 g/L (p = 0.05) treatments. HAMP expression showed alternating elevations and increased upon 1 g/L (p < 0.05) and 5 g/L (p < 0.05). However, in the recombinant cells that showed higher intracellular iron levels than wild-type cells, HFE and HAMP expressions were elevated only at low 1 g/L treatment (p < 0.03) and were repressed at 2 g/L treatment (p < 0.03). Under holotransferrin-untreated conditions, the iron-loaded recombinant cells showed higher expressions of HFE (p < 0.03) and HAMP (p = 0.05) than wild-type cells. HFE mRNA was independently elevated by extracellular and intracellular iron-excess. Thus, it may be involved in sensing both, extracellular and intracellular iron. Repression of HAMP expression under simultaneous intracellular and extracellular iron-loading resembles non-hereditary iron-excess pathologies.

KW - Antigens, CD/metabolism

KW - Cells, Cultured

KW - Gene Expression Regulation

KW - Hemochromatosis/genetics

KW - Hemochromatosis Protein/genetics

KW - Hep G2 Cells

KW - Hepatocytes/metabolism

KW - Hepcidins/genetics

KW - Histocompatibility Antigens Class I/genetics

KW - Homeostasis

KW - Humans

KW - Iron/metabolism

KW - Liver/metabolism

KW - RNA, Messenger/genetics

KW - Real-Time Polymerase Chain Reaction

KW - Receptors, Transferrin/metabolism

KW - Transferrin/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85028336862&partnerID=8YFLogxK

U2 - 10.1007/s11033-017-4123-2

DO - 10.1007/s11033-017-4123-2

M3 - Article

C2 - 28840425

VL - 44

SP - 399

EP - 403

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 5

ER -

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