High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration

R. G. Kay; C. Barton; C. P. Velloso; P. R. Brown; C. Bartlett; A. J. Blazevich; R. J. Godfrey; G. Goldspink; R. Rees; G. R. Ball; D. A. Cowan; S. D. Harridge; J. Roberts; P. Teale; C. S. Creaser

doi:10.1002/rcm.4237

High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration

R. G. Kay, C. Barton, C. P. Velloso, P. R. Brown, C. Bartlett, A. J. Blazevich, R. J. Godfrey, G. Goldspink, R. Rees, G. R. Ball, D. A. Cowan, S. D. Harridge, J. Roberts, P. Teale, C. S. Creaser

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Insulin-like growth factor-I (IGF-I) is a known biomarker of recombinant human growth hormone (rhGH) abuse, and is also used clinically to confirm acromegaly. The protein leucine-rich alpha-2-glycoprotein (LRG) was recently identified as a putative biomarker of rhGH administration. The combination of an ACN depletion method and a 5-min ultra-high-performance liquid chromatography/tandem mass spectrometry (uHPLC/MS/MS)-based selected reaction monitoring (SRM) assay detected both IGF-I and LRG at endogenous concentrations. Four eight-point standard addition curves of IGF-I (16-2000 ng/mL) demonstrated good linearity (r(2) = 0.9991 and coefficients of variance (CVs)

Original language	English
Pages (from-to)	3173 - 3182
Number of pages	10
Journal	Rapid Communications in Mass Spectrometry
Volume	23
Issue number	19
DOIs	https://doi.org/10.1002/rcm.4237
Publication status	Published - 15 Oct 2009

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Kay, R. G., Barton, C., Velloso, C. P., Brown, P. R., Bartlett, C., Blazevich, A. J., Godfrey, R. J., Goldspink, G., Rees, R., Ball, G. R., Cowan, D. A., Harridge, S. D., Roberts, J., Teale, P., & Creaser, C. S. (2009). High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration. Rapid Communications in Mass Spectrometry, 23(19), 3173 - 3182. https://doi.org/10.1002/rcm.4237

Kay, R. G. ; Barton, C. ; Velloso, C. P. et al. / High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration. In: Rapid Communications in Mass Spectrometry. 2009 ; Vol. 23, No. 19. pp. 3173 - 3182.

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title = "High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration",

abstract = "Insulin-like growth factor-I (IGF-I) is a known biomarker of recombinant human growth hormone (rhGH) abuse, and is also used clinically to confirm acromegaly. The protein leucine-rich alpha-2-glycoprotein (LRG) was recently identified as a putative biomarker of rhGH administration. The combination of an ACN depletion method and a 5-min ultra-high-performance liquid chromatography/tandem mass spectrometry (uHPLC/MS/MS)-based selected reaction monitoring (SRM) assay detected both IGF-I and LRG at endogenous concentrations. Four eight-point standard addition curves of IGF-I (16-2000 ng/mL) demonstrated good linearity (r(2) = 0.9991 and coefficients of variance (CVs) ",

author = "Kay, {R. G.} and C. Barton and Velloso, {C. P.} and Brown, {P. R.} and C. Bartlett and Blazevich, {A. J.} and Godfrey, {R. J.} and G. Goldspink and R. Rees and Ball, {G. R.} and Cowan, {D. A.} and Harridge, {S. D.} and J. Roberts and P. Teale and Creaser, {C. S.}",

year = "2009",

month = oct,

day = "15",

doi = "10.1002/rcm.4237",

language = "English",

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Kay, RG, Barton, C, Velloso, CP, Brown, PR, Bartlett, C, Blazevich, AJ, Godfrey, RJ, Goldspink, G, Rees, R, Ball, GR, Cowan, DA, Harridge, SD, Roberts, J, Teale, P & Creaser, CS 2009, 'High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration', Rapid Communications in Mass Spectrometry, vol. 23, no. 19, pp. 3173 - 3182. https://doi.org/10.1002/rcm.4237

High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration. / Kay, R. G.; Barton, C.; Velloso, C. P. et al.
In: Rapid Communications in Mass Spectrometry, Vol. 23, No. 19, 15.10.2009, p. 3173 - 3182.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration

AU - Kay, R. G.

AU - Barton, C.

AU - Velloso, C. P.

AU - Brown, P. R.

AU - Bartlett, C.

AU - Blazevich, A. J.

AU - Godfrey, R. J.

AU - Goldspink, G.

AU - Rees, R.

AU - Ball, G. R.

AU - Cowan, D. A.

AU - Harridge, S. D.

AU - Roberts, J.

AU - Teale, P.

AU - Creaser, C. S.

PY - 2009/10/15

Y1 - 2009/10/15

N2 - Insulin-like growth factor-I (IGF-I) is a known biomarker of recombinant human growth hormone (rhGH) abuse, and is also used clinically to confirm acromegaly. The protein leucine-rich alpha-2-glycoprotein (LRG) was recently identified as a putative biomarker of rhGH administration. The combination of an ACN depletion method and a 5-min ultra-high-performance liquid chromatography/tandem mass spectrometry (uHPLC/MS/MS)-based selected reaction monitoring (SRM) assay detected both IGF-I and LRG at endogenous concentrations. Four eight-point standard addition curves of IGF-I (16-2000 ng/mL) demonstrated good linearity (r(2) = 0.9991 and coefficients of variance (CVs)

AB - Insulin-like growth factor-I (IGF-I) is a known biomarker of recombinant human growth hormone (rhGH) abuse, and is also used clinically to confirm acromegaly. The protein leucine-rich alpha-2-glycoprotein (LRG) was recently identified as a putative biomarker of rhGH administration. The combination of an ACN depletion method and a 5-min ultra-high-performance liquid chromatography/tandem mass spectrometry (uHPLC/MS/MS)-based selected reaction monitoring (SRM) assay detected both IGF-I and LRG at endogenous concentrations. Four eight-point standard addition curves of IGF-I (16-2000 ng/mL) demonstrated good linearity (r(2) = 0.9991 and coefficients of variance (CVs)

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DO - 10.1002/rcm.4237

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JF - Rapid Communications in Mass Spectrometry

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Kay RG, Barton C, Velloso CP, Brown PR, Bartlett C, Blazevich AJ et al. High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration. Rapid Communications in Mass Spectrometry. 2009 Oct 15;23(19):3173 - 3182. doi: 10.1002/rcm.4237