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HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection

Research output: Contribution to journalArticle

Candida F. Pereira, Jeremie Rossy, Dylan Owen, Johnson Mak, Katharina Gaus

Original languageEnglish
Article number84
Pages (from-to)-
Number of pages6
JournalJournal of Medical Virology
Volume9
DOIs
Published2 May 2012

King's Authors

Abstract

Background: The visualization of viral proteins has been hindered by the resolution limit of conventional fluorescent microscopes, as the dimension of any single fluorescent signal is often greater than most virion particles. Super-resolution microscopy has the potential to unveil the distribution of proteins at the resolution approaching electron microscopy without relying on morphological features of existing characteristics of the biological specimen that are needed in EM.

Results: Using direct stochastic optical reconstruction microscopy (dSTORM) to achieve a lateral resolution of 1520 nm, we quantified the 2-D molecular distribution of the major structural proteins of the infectious human immunodeficiency virus type 1 (HIV-1) before and after infection of lymphoid cells. We determined that the HIV-1 matrix and capsid proteins undergo restructuring soon after HIV-1 infection.

Conclusions: This study provides the proof-of-concept for the use of dSTORM to visualize the changes in the molecular distribution of viral proteins during an infection.

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