How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology

Gilbert O. Fruhwirth; Luis P. Fernandes; Gregory Weitsman; Gargi Patel; Muireann Kelleher; Katherine Lawler; Adrian Brock; Simon P. Poland; Daniel R. Matthews; Gyoergy Keri; Paul R. Barber; Borivoj Vojnovic; Simon M. Ameer-Beg; Anthony C. C. Coolen; Franca Fraternali; Tony Ng

doi:10.1002/cphc.201000866

How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology

Gilbert O. Fruhwirth, Luis P. Fernandes, Gregory Weitsman, Gargi Patel, Muireann Kelleher, Katherine Lawler, Adrian Brock, Simon P. Poland, Daniel R. Matthews, Gyoergy Keri, Paul R. Barber, Borivoj Vojnovic, Simon M. Ameer-Beg, Anthony C. C. Coolen, Franca Fraternali, Tony Ng

Research output: Contribution to journal › Literature review › peer-review

45 Citations (Scopus)

Abstract

Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits ( nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.

Original language	English
Pages (from-to)	442 - 461
Number of pages	20
Journal	Chemphyschem : A European journal of chemical physics and physical chemistry
Volume	12
Issue number	3
DOIs	https://doi.org/10.1002/cphc.201000866
Publication status	Published - 25 Feb 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/cphc.201000866

Cite this

Fruhwirth, G. O., Fernandes, L. P., Weitsman, G., Patel, G., Kelleher, M., Lawler, K., Brock, A., Poland, S. P., Matthews, D. R., Keri, G., Barber, P. R., Vojnovic, B., Ameer-Beg, S. M., Coolen, A. C. C., Fraternali, F., & Ng, T. (2011). How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology. Chemphyschem : A European journal of chemical physics and physical chemistry, 12(3), 442 - 461. https://doi.org/10.1002/cphc.201000866

@article{abc922f8f13f449ba911257d43c0d78a,

title = "How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology",

abstract = "Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits ( nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.",

author = "Fruhwirth, {Gilbert O.} and Fernandes, {Luis P.} and Gregory Weitsman and Gargi Patel and Muireann Kelleher and Katherine Lawler and Adrian Brock and Poland, {Simon P.} and Matthews, {Daniel R.} and Gyoergy Keri and Barber, {Paul R.} and Borivoj Vojnovic and Ameer-Beg, {Simon M.} and Coolen, {Anthony C. C.} and Franca Fraternali and Tony Ng",

year = "2011",

month = feb,

day = "25",

doi = "10.1002/cphc.201000866",

language = "English",

volume = "12",

pages = "442 -- 461",

journal = "Chemphyschem : A European journal of chemical physics and physical chemistry",

publisher = "Wiley-Blackwell",

number = "3",

}

Fruhwirth, GO, Fernandes, LP, Weitsman, G , Patel, G, Kelleher, M, Lawler, K, Brock, A, Poland, SP , Matthews, DR, Keri, G, Barber, PR, Vojnovic, B , Ameer-Beg, SM , Coolen, ACC , Fraternali, F & Ng, T 2011, 'How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology', Chemphyschem : A European journal of chemical physics and physical chemistry, vol. 12, no. 3, pp. 442 - 461. https://doi.org/10.1002/cphc.201000866

How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology. / Fruhwirth, Gilbert O.; Fernandes, Luis P.; Weitsman, Gregory et al.
In: Chemphyschem : A European journal of chemical physics and physical chemistry, Vol. 12, No. 3, 25.02.2011, p. 442 - 461.

Research output: Contribution to journal › Literature review › peer-review

TY - JOUR

T1 - How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology

AU - Fruhwirth, Gilbert O.

AU - Fernandes, Luis P.

AU - Weitsman, Gregory

AU - Patel, Gargi

AU - Kelleher, Muireann

AU - Lawler, Katherine

AU - Brock, Adrian

AU - Poland, Simon P.

AU - Matthews, Daniel R.

AU - Keri, Gyoergy

AU - Barber, Paul R.

AU - Vojnovic, Borivoj

AU - Ameer-Beg, Simon M.

AU - Coolen, Anthony C. C.

AU - Fraternali, Franca

AU - Ng, Tony

PY - 2011/2/25

Y1 - 2011/2/25

N2 - Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits ( nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.

AB - Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits ( nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.

U2 - 10.1002/cphc.201000866

DO - 10.1002/cphc.201000866

M3 - Literature review

VL - 12

SP - 442

EP - 461

JO - Chemphyschem : A European journal of chemical physics and physical chemistry

JF - Chemphyschem : A European journal of chemical physics and physical chemistry

IS - 3

ER -

How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this