Human pluripotent stem cells: An alternative for 3D in vitro modelling of skin disease

Preeti Khurana*, Nikola Kolundzic, Carsten Flohr, Dusko Ilic

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)

Abstract

To effectively study the skin and its pathology, various platforms have been used to date, with in vitro 3D skin models being considered the future gold standard. These models have generally been engineered from primary cell lines. However, their short life span leading to the use of various donors, imposes issues with genetic variation. Human pluripotent stem cell (hPSC)-technology holds great prospects as an alternative to the use of primary cell lines to study the pathophysiology of human skin diseases. This is due to their potential to generate an unlimited number of genetically identical skin models that closely mimic the complexity of in vivo human skin. During the past decade, researchers have therefore started to use human embryonic and induced pluripotent stem cells (hESC/iPSC) to derive skin resident-like cells and components. These have subsequently been used to engineer hPSC-derived 3D skin models. In this review, we focus on the advantages, recent developments, and future perspectives in using hPSCs as an alternative cell source for modelling human skin diseases in vitro.

Original languageEnglish
Pages (from-to)1572-1587
Number of pages16
JournalExperimental Dermatology
Volume30
Issue number11
DOIs
Publication statusPublished - Nov 2021

Keywords

  • 3D skin
  • differentiation
  • human model
  • keratinocytes
  • stem cells

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