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Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration

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Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. / Bai, Siau Wei; Herrera-Abreu, Maria Teresa; Rohn, Jennifer L; Racine, Victor; Tajadura, Virginia; Suryavanshi, Narendra; Bechtel, Stephanie; Wiemann, Stefan; Baum, Buzz; Ridley, Anne J.

In: BMC Biology, Vol. 9, 54, 11.08.2011.

Research output: Contribution to journalArticle

Harvard

Bai, SW, Herrera-Abreu, MT, Rohn, JL, Racine, V, Tajadura, V, Suryavanshi, N, Bechtel, S, Wiemann, S, Baum, B & Ridley, AJ 2011, 'Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration', BMC Biology, vol. 9, 54. https://doi.org/10.1186/1741-7007-9-54

APA

Bai, S. W., Herrera-Abreu, M. T., Rohn, J. L., Racine, V., Tajadura, V., Suryavanshi, N., Bechtel, S., Wiemann, S., Baum, B., & Ridley, A. J. (2011). Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biology, 9, [54]. https://doi.org/10.1186/1741-7007-9-54

Vancouver

Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N et al. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biology. 2011 Aug 11;9. 54. https://doi.org/10.1186/1741-7007-9-54

Author

Bai, Siau Wei ; Herrera-Abreu, Maria Teresa ; Rohn, Jennifer L ; Racine, Victor ; Tajadura, Virginia ; Suryavanshi, Narendra ; Bechtel, Stephanie ; Wiemann, Stefan ; Baum, Buzz ; Ridley, Anne J. / Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. In: BMC Biology. 2011 ; Vol. 9.

Bibtex Download

@article{e636cfbdbe664e8891a5e3e96d50c767,
title = "Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration",
abstract = "BACKGROUND: Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways.RESULTS: Here we use data from a genome-wide RNAi morphology screen in Drosophila melanogaster cells together with bioinformatics to identify 26 new regulators of morphology and cytoskeletal organization in human cells. These include genes previously implicated in a wide range of functions, from mental retardation, Down syndrome and Huntington's disease to RNA and DNA-binding genes. We classify these genes into seven groups according to phenotype and identify those that affect cell migration. We further characterize a subset of seven genes, FAM40A, FAM40B, ARC, FMNL3, FNBP3/FBP11, LIMD1 and ZRANB1, each of which has a different effect on cell shape, actin filament distribution and cell migration. Interestingly, in several instances closely related isoforms with a single Drosophila homologue have distinct phenotypes. For example, FAM40B depletion induces cell elongation and tail retraction defects, whereas FAM40A depletion reduces cell spreading.CONCLUSIONS: Our results identify multiple regulators of cell migration and cytoskeletal signalling that are highly conserved between Drosophila and humans, and show that closely related paralogues can have very different functions in these processes.",
keywords = "Actin Cytoskeleton, Actins, Animals, Cell Movement, Cell Shape, Conserved Sequence, Drosophila Proteins, Drosophila melanogaster, Genes, Insect, Genetic Testing, HeLa Cells, Humans, Mice, NIH 3T3 Cells, Phenotype, Proteins, RNA, Small Interfering, Wound Healing",
author = "Bai, {Siau Wei} and Herrera-Abreu, {Maria Teresa} and Rohn, {Jennifer L} and Victor Racine and Virginia Tajadura and Narendra Suryavanshi and Stephanie Bechtel and Stefan Wiemann and Buzz Baum and Ridley, {Anne J}",
year = "2011",
month = aug,
day = "11",
doi = "10.1186/1741-7007-9-54",
language = "English",
volume = "9",
journal = "BMC Biology",
issn = "1741-7007",
publisher = "BioMed Central",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration

AU - Bai, Siau Wei

AU - Herrera-Abreu, Maria Teresa

AU - Rohn, Jennifer L

AU - Racine, Victor

AU - Tajadura, Virginia

AU - Suryavanshi, Narendra

AU - Bechtel, Stephanie

AU - Wiemann, Stefan

AU - Baum, Buzz

AU - Ridley, Anne J

PY - 2011/8/11

Y1 - 2011/8/11

N2 - BACKGROUND: Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways.RESULTS: Here we use data from a genome-wide RNAi morphology screen in Drosophila melanogaster cells together with bioinformatics to identify 26 new regulators of morphology and cytoskeletal organization in human cells. These include genes previously implicated in a wide range of functions, from mental retardation, Down syndrome and Huntington's disease to RNA and DNA-binding genes. We classify these genes into seven groups according to phenotype and identify those that affect cell migration. We further characterize a subset of seven genes, FAM40A, FAM40B, ARC, FMNL3, FNBP3/FBP11, LIMD1 and ZRANB1, each of which has a different effect on cell shape, actin filament distribution and cell migration. Interestingly, in several instances closely related isoforms with a single Drosophila homologue have distinct phenotypes. For example, FAM40B depletion induces cell elongation and tail retraction defects, whereas FAM40A depletion reduces cell spreading.CONCLUSIONS: Our results identify multiple regulators of cell migration and cytoskeletal signalling that are highly conserved between Drosophila and humans, and show that closely related paralogues can have very different functions in these processes.

AB - BACKGROUND: Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways.RESULTS: Here we use data from a genome-wide RNAi morphology screen in Drosophila melanogaster cells together with bioinformatics to identify 26 new regulators of morphology and cytoskeletal organization in human cells. These include genes previously implicated in a wide range of functions, from mental retardation, Down syndrome and Huntington's disease to RNA and DNA-binding genes. We classify these genes into seven groups according to phenotype and identify those that affect cell migration. We further characterize a subset of seven genes, FAM40A, FAM40B, ARC, FMNL3, FNBP3/FBP11, LIMD1 and ZRANB1, each of which has a different effect on cell shape, actin filament distribution and cell migration. Interestingly, in several instances closely related isoforms with a single Drosophila homologue have distinct phenotypes. For example, FAM40B depletion induces cell elongation and tail retraction defects, whereas FAM40A depletion reduces cell spreading.CONCLUSIONS: Our results identify multiple regulators of cell migration and cytoskeletal signalling that are highly conserved between Drosophila and humans, and show that closely related paralogues can have very different functions in these processes.

KW - Actin Cytoskeleton

KW - Actins

KW - Animals

KW - Cell Movement

KW - Cell Shape

KW - Conserved Sequence

KW - Drosophila Proteins

KW - Drosophila melanogaster

KW - Genes, Insect

KW - Genetic Testing

KW - HeLa Cells

KW - Humans

KW - Mice

KW - NIH 3T3 Cells

KW - Phenotype

KW - Proteins

KW - RNA, Small Interfering

KW - Wound Healing

U2 - 10.1186/1741-7007-9-54

DO - 10.1186/1741-7007-9-54

M3 - Article

C2 - 21834987

VL - 9

JO - BMC Biology

JF - BMC Biology

SN - 1741-7007

M1 - 54

ER -

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