Identification and characterization of the surface-layer protein of Clostridium tetani

Omar Qazi; Alan Brailsford; Anne Wright; Jeremy Faraar; Jim Campbell; Neil Fairweather

doi:10.1111/j.1574-6968.2007.00834.x

Identification and characterization of the surface-layer protein of Clostridium tetani

Omar Qazi, Alan Brailsford, Anne Wright, Jeremy Faraar, Jim Campbell, Neil Fairweather

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Many bacterial species produce a paracrystalline layer, the surface layer, which completely surrounds the exterior of the cell. In some bacteria, the surface layer is implicated in pathogenesis. Two proteins present in cell wall extracts from Clostridium tetani have been investigated and identified one of these has been unambiguously as the surface-layer protein (SLP). The gene, slpA, has been located in the genome of C. tetani E88 that encodes the SLP. The molecular mass of the protein as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is considerably larger than that predicted from the gene; however the protein does not appear to be glycosylated. Furthermore, analysis of five C. tetani strains, including three recent clinical isolates, shows considerable variation in the sizes of the SLP.

Original language	English
Pages (from-to)	126-31
Number of pages	6
Journal	Fems Microbiology Letters
Volume	274
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00834.x
Publication status	Published - Sept 2007

Keywords

Mass Spectrometry
Genetic Variation
Genome, Bacterial
Amino Acid Motifs
Electrophoresis, Polyacrylamide Gel
Glycosylation
Bacterial Outer Membrane Proteins
Clostridium tetani

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10.1111/j.1574-6968.2007.00834.x

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title = "Identification and characterization of the surface-layer protein of Clostridium tetani",

abstract = "Many bacterial species produce a paracrystalline layer, the surface layer, which completely surrounds the exterior of the cell. In some bacteria, the surface layer is implicated in pathogenesis. Two proteins present in cell wall extracts from Clostridium tetani have been investigated and identified one of these has been unambiguously as the surface-layer protein (SLP). The gene, slpA, has been located in the genome of C. tetani E88 that encodes the SLP. The molecular mass of the protein as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is considerably larger than that predicted from the gene; however the protein does not appear to be glycosylated. Furthermore, analysis of five C. tetani strains, including three recent clinical isolates, shows considerable variation in the sizes of the SLP.",

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T1 - Identification and characterization of the surface-layer protein of Clostridium tetani

AU - Qazi, Omar

AU - Brailsford, Alan

AU - Wright, Anne

AU - Faraar, Jeremy

AU - Campbell, Jim

AU - Fairweather, Neil

PY - 2007/9

Y1 - 2007/9

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AB - Many bacterial species produce a paracrystalline layer, the surface layer, which completely surrounds the exterior of the cell. In some bacteria, the surface layer is implicated in pathogenesis. Two proteins present in cell wall extracts from Clostridium tetani have been investigated and identified one of these has been unambiguously as the surface-layer protein (SLP). The gene, slpA, has been located in the genome of C. tetani E88 that encodes the SLP. The molecular mass of the protein as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is considerably larger than that predicted from the gene; however the protein does not appear to be glycosylated. Furthermore, analysis of five C. tetani strains, including three recent clinical isolates, shows considerable variation in the sizes of the SLP.

KW - Mass Spectrometry

KW - Genetic Variation

KW - Genome, Bacterial

KW - Amino Acid Motifs

KW - Electrophoresis, Polyacrylamide Gel

KW - Glycosylation

KW - Bacterial Outer Membrane Proteins

KW - Clostridium tetani

U2 - 10.1111/j.1574-6968.2007.00834.x

DO - 10.1111/j.1574-6968.2007.00834.x

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EP - 131

JO - Fems Microbiology Letters

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Identification and characterization of the surface-layer protein of Clostridium tetani

Abstract

Keywords

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