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Identification of an intestinal heme transporter

Research output: Contribution to journalArticle

M Shayeghi, G Latunde-Dada, J Oakhill, K Takeuchi, A Laftah, N Halliday, Y Khan, A Warley, F McCann, R Hider, D Frazer, G Anderson, C Vulpe, R Simpson, A McKie

Original languageEnglish
Pages (from-to)789 - 801
Number of pages13
JournalCell
Volume122
Issue number5
DOIs
Publication statusPublished - 9 Sep 2005

King's Authors

Impacts

  • BBC News story about HCP1

    Impact: Health Impacts

Abstract

Dietary heme iron is an important nutritional source of iron in carnivores and omnivores that is more readily absorbed than non-heme iron derived from vegetables and grain. Most heme is absorbed in the proximal intestine, with absorptive capacity decreasing distally. We utilized a subtractive hybridization approach to isolate a heme transporter from duodenum by taking advantage of the intestinal gradient for heme absorption. Here we show a membrane protein named HCP1 (heme carrier protein 1), with homology to bacterial metal-tetracycIine transporters, mediates heme uptake by cells in a temperature-dependent and saturable manner. HCP1 mRNA was highly expressed in duodenum and regulated by hypoxia. HCP1 protein was iron regulated and localized to the brush-border membrane of duodenal enterocytes in iron deficiency

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