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Identification of functional alpha(2)- and beta-adrenergic receptors in mammalian spermatozoa

Research output: Contribution to journalArticlepeer-review

S A Adeoya-Osiguwa, R Gibbons, L R Fraser

Original languageEnglish
Pages (from-to)1555 - 1563
Number of pages9
JournalHuman Reproduction
Volume21
Issue number6
DOIs
PublishedJun 2006

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Research Groups

  • King's College London

Abstract

BACKGROUND: A recent study of several compounds, structurally related to amphetamine, provided evidence that mammalian spermatozoa might have adrenergic receptors able to regulate cAMP production. The present study investigated this possibility using physiological and immunochemical analyses of mouse and human spermatozoa. METHODS: Antibodies specific for different receptor subtypes were used for Western blotting of mouse and human sperm lysates and for immunocytochemical evaluation of whole mouse and human spermatozoa. Uncapacitated and capacitated mouse spermatozoa were incubated with specific agonists and antagonists for alpha(2)-, beta(1)-, beta(2)- and beta(3)-adrenergic receptors for similar to 35 min and then assessed using chlortetracycline (CTC) fluorescence. RESULTS: Western blotting revealed proteins of the correct size for all these receptors; immunolocalization indicated their presence on the head, especially acrosomal and neck regions, and flagellum of both mouse and human spermatozoa. CTC results indicated significant responses to agonists for all of the beta-receptors in uncapacitated cells, with agonist effectiveness being beta(1) > beta(2) > beta(3); relevant antagonists blocked responses. In contrast, an agonist and antagonist for alpha(2)-receptors acted only on capacitated spermatozoa. CONCLUSION: These experiments provide the first good evidence that mammalian spermatozoa have both beta-adrenergic receptors, known to stimulate cAMP production by membrane-associated adenylyl cyclases (mACs), and alpha(2)-adrenergic receptors, known to inhibit cAMP production by mACs. Responses are capacitation state dependent and provide a mechanism for inhibiting spontaneous acrosome reactions and helping to maintain fertilizing ability. These results suggest that the use of amphetamine-related compounds, either for medical or for social reasons, might have an unexpected positive impact on fertility

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