Projects per year
The expression of anti-inflammatory IL-10 by CD4 + T cells is indispensable for immune homeostasis, as it allows T cells to moderate their effector function. We previously showed that TNF-a blockade during T cell stimulation in CD4 + T cell/monocyte cocultures resulted in maintenance of IL-10–producing T cells and identified IKZF3 as a putative regulator of IL-10. In this study, we tested the hypothesis that IKZF3 is a transcriptional regulator of IL-10 using a human CD4 + T cell–only culture system. IL-10 + CD4 + T cells expressed the highest levels of IKZF3 both ex vivo and after activation compared with IL-10–CD4 + T cells. Pharmacological targeting of IKZF3 with the drug lenalidomide showed that IKZF3 is required for anti-CD3/CD28 mAb–mediated induction of IL-10 but is dispensable for ex vivo IL-10 expression. However, overexpression of IKZF3 was unable to upregulate IL-10 at the mRNA or protein level in CD4 + T cells and did not drive the transcription of the IL10 promoter or putative local enhancer constructs. Collectively, these data indicate that IKZF3 is associated with but not sufficient for IL-10 expression in CD4 + T cells.
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- 3 Finished
Determining the molecular mechanisms underlying IL-10 expression in human CD4+ T-cells following TNF blockade
1/04/2016 → 31/07/2023
Afzali, B., Antoniou, M., Barker, J., Barratt, B., Beighton, D., Bevan, S., Blower, P., Burchell, J., Cason, J., Chappell, L., Cope, A., Corrigan, C., Cunninghame Graham, D., Dontu, G., Edgeworth, J., Farzaneh, F., Gee, T., Gould, H., Green, P., Greenough, A., Grigoriadis, A., Hayday, A., Higginson, I., Kalra, L., Karagiannis, S., Kelly, F., Kemper, C., Lau-Walker, M., Lavender, P., Lee, T., Lempp, H., Lewis, C., Lombardi, G., Lord, G., Marber, M., Mason, J., Mathew, C., McGrath, J., Metcalfe, A., Molokhia, M., Mullen, G., Nagel, E., Neil, S., Nestle, F., Oakey, R., Parker, P., Peakman, M., Perera, D., Purushotham, A., Rasekh Ahmadi, K., Rashid, T., Razavi, R., Ridley, A., Robson, M., Sacks, S., Sanchez Fueyo, A., Santis, G., Sanz Moreno, V., Sawyer, E., Schaeffter, T., Shah, A., Sharpe, P., Simpson, M., Smith, R., Sorinola, I., Spector, T., Spicer, J., Taams, L., Tavassoli, M., Taylor, D., Tree, T., Trembath, R., Tribe, R., Tutt, A., Waltham, M., Watt, F., Webb, A., Wells, C., Whelan, K., Whitaker, J., Whittlesea, C., Williamson, C., Wolfe, C., Yu, V., academic, A., Hughes, S., Montana, G., Rhode, K., Schnabel, J. & Tziotzios, C.
1/04/2007 → 31/03/2017