Image cytometry DNA ploidy analysis: correlation between two semi-automated methods

TY - JOUR

T1 - Image cytometry DNA ploidy analysis

T2 - correlation between two semi-automated methods

AU - Ghizoni, Janaina Salomon

AU - Sperandio, Marcelo

AU - Lock, Claire

AU - Odell, Edward W

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Background and Objectives Quantitation of cell DNA content, DNA ploidy, has been established as a research and prognostic technique for decades. A variety of instruments have been used although only a few commercially available systems have established quality assurance and published outcome data. The aim of this study was to compare two automated systems. Methods Nuclear monolayers were obtained from 112 oral biopsies by enzyme digestion and Feulgen staining. These were scanned on both the Fairfield and the Ploidy Work Station (PWS) systems. The overall ploidy diagnosis, number of epithelial nuclei, coefficient of variation (CV) and 5c exceeding rate (5CER) were compared by quantile–quantile plots, t test, Wilcoxon and Spearman's tests. Results The PWS system identified more nuclei (p < 0.0001) at a lower CV (p < 0.0001). Using the PWS system, fewer samples were classified as indeterminate. No difference between 5CER was found between systems (p > 0.54). There was complete concordance between the two systems in terms of DNA ploidy diagnosis. Conclusions The PWS system is comparable to the Fairfield system for determination of DNA ploidy and has advantages that may lead to improved performance.

AB - Background and Objectives Quantitation of cell DNA content, DNA ploidy, has been established as a research and prognostic technique for decades. A variety of instruments have been used although only a few commercially available systems have established quality assurance and published outcome data. The aim of this study was to compare two automated systems. Methods Nuclear monolayers were obtained from 112 oral biopsies by enzyme digestion and Feulgen staining. These were scanned on both the Fairfield and the Ploidy Work Station (PWS) systems. The overall ploidy diagnosis, number of epithelial nuclei, coefficient of variation (CV) and 5c exceeding rate (5CER) were compared by quantile–quantile plots, t test, Wilcoxon and Spearman's tests. Results The PWS system identified more nuclei (p < 0.0001) at a lower CV (p < 0.0001). Using the PWS system, fewer samples were classified as indeterminate. No difference between 5CER was found between systems (p > 0.54). There was complete concordance between the two systems in terms of DNA ploidy diagnosis. Conclusions The PWS system is comparable to the Fairfield system for determination of DNA ploidy and has advantages that may lead to improved performance.

U2 - 0.1111/odi.12888

DO - 0.1111/odi.12888

M3 - Article

SN - 1354-523X

VL - 24

SP - 1204

EP - 1208

JO - Oral Diseases

JF - Oral Diseases

IS - 7

ER -