Imaging Cells at the Nanoscale

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21 Citations (Scopus)

Abstract

Recently developed super-resolution techniques in optical microscopy have pushed the length scale at which cellular structure can be observed down to tens of nanometres. A wide array of methods have been described that fall under the umbrella term of super-resolution microscopy and each of these methods has different requirements for acquisition speed, experimental complexity, fluorophore requirements and post-processing of data. For example, experimental complexity can be decreased by using a standard widefield microscope for acquisition, but this requires substantial processing of the data to extract the super-resolution information.

These powerful techniques are bringing new insights into the nanoscale structure of sub-cellular assemblies such as podosomes, which are an ideal system to observe with super-resolution microscopy as the structures are relatively thin and they form and dissociate over a period of several minutes. Here we discuss the major classes of super-resolution microscopy techniques, and demonstrate their relative performance by imaging podosomes.
Original languageEnglish
Article numberN/A
Pages (from-to)1669-1678
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume45
Issue number8
DOIs
Publication statusPublished - Aug 2013

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