Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

Ida Emilie Steinmark, Arjuna L. James, Cecile Ayako Dreiss, Gokhan Yahioglu, Klaus Suhling

Research output: Chapter in Book/Report/Conference proceedingConference paper

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Abstract

Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI
EditorsSamuel Achilefu, Ramesh Raghavachari
PublisherSPIE
Volume10893
ISBN (Electronic)9781510624283
DOIs
Publication statusPublished - 7 Mar 2019

Keywords

  • Fluorescence lifetime imaging
  • Fluorescent molecular rotors
  • Mitochondria
  • Viscosity

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