Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

Ida Emilie Steinmark; Arjuna L. James; Cecile Ayako Dreiss; Gokhan Yahioglu; Klaus Suhling

doi:10.1117/12.2508676

Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

Ida Emilie Steinmark
, Arjuna L. James
, Cecile Ayako Dreiss
, Gokhan Yahioglu
, Klaus Suhling

Research output: Chapter in Book/Report/Conference proceeding › Conference paper

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Abstract

Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

Original language	English
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI
Editors	Samuel Achilefu, Ramesh Raghavachari
Publisher	SPIE
Volume	10893
ISBN (Electronic)	9781510624283
DOIs	https://doi.org/10.1117/12.2508676
Publication status	Published - 7 Mar 2019

Keywords

Fluorescence lifetime imaging
Fluorescent molecular rotors
Mitochondria
Viscosity

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10.1117/12.2508676

Imaging mitochondrial matrix viscosity_STEINMARK_Firstonline7March2019_GREEN AAMAccepted author manuscript, 1.42 MB

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Steinmark, I. E., James, A. L., Dreiss, C. A., Yahioglu, G., & Suhling, K. (2019). Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors. In S. Achilefu, & R. Raghavachari (Eds.), Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI (Vol. 10893). Article 108930K SPIE. https://doi.org/10.1117/12.2508676

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abstract = "Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.",

keywords = "Fluorescence lifetime imaging, Fluorescent molecular rotors, Mitochondria, Viscosity",

author = "Steinmark, \{Ida Emilie\} and James, \{Arjuna L.\} and Dreiss, \{Cecile Ayako\} and Gokhan Yahioglu and Klaus Suhling",

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doi = "10.1117/12.2508676",

language = "English",

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Steinmark, IE, James, AL, Dreiss, CA, Yahioglu, G & Suhling, K 2019, Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors. in S Achilefu & R Raghavachari (eds), Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI. vol. 10893, 108930K, SPIE. https://doi.org/10.1117/12.2508676

Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors. / Steinmark, Ida Emilie; James, Arjuna L.; Dreiss, Cecile Ayako et al.
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI. ed. / Samuel Achilefu; Ramesh Raghavachari. Vol. 10893 SPIE, 2019. 108930K.

Research output: Chapter in Book/Report/Conference proceeding › Conference paper

TY - CHAP

T1 - Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

AU - Steinmark, Ida Emilie

AU - James, Arjuna L.

AU - Dreiss, Cecile Ayako

AU - Yahioglu, Gokhan

AU - Suhling, Klaus

PY - 2019/3/7

Y1 - 2019/3/7

N2 - Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

AB - Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

KW - Fluorescence lifetime imaging

KW - Fluorescent molecular rotors

KW - Mitochondria

KW - Viscosity

UR - https://www.scopus.com/pages/publications/85073778750

U2 - 10.1117/12.2508676

DO - 10.1117/12.2508676

M3 - Conference paper

VL - 10893

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI

A2 - Achilefu, Samuel

A2 - Raghavachari, Ramesh

PB - SPIE

ER -

Steinmark IE, James AL, Dreiss CA, Yahioglu G, Suhling K. Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors. In Achilefu S, Raghavachari R, editors, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI. Vol. 10893. SPIE. 2019. 108930K doi: 10.1117/12.2508676

Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

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Keywords

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