Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering

Molly Lowndes; Michael Rotherham; Joshua C. Price; Alicia J. El Haj; Shukry J. Habib

doi:10.1016/j.stemcr.2016.06.004

Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering

Molly Lowndes, Michael Rotherham, Joshua C. Price, Alicia J. El Haj, Shukry J. Habib

Centre for Stem Cells & Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

145 Downloads (Pure)

Abstract

Summary:
The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

Original language	English
Pages (from-to)	126-137
Number of pages	12
Journal	Stem cell reports
Volume	7
Issue number	1
Early online date	12 Jul 2016
DOIs	https://doi.org/10.1016/j.stemcr.2016.06.004
Publication status	Published - 12 Jul 2016

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10.1016/j.stemcr.2016.06.004Licence: CC BY

Immobilized WNT Proteins Act_LOWNDES_Accepted8June2016_GOLD VoRFinal published version, 1.86 MBLicence: CC BY

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AU - Rotherham, Michael

AU - Price, Joshua C.

AU - El Haj, Alicia J.

AU - Habib, Shukry J.

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AB - Summary:The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

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Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering

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