Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women

Giulia Piaggeschi; Simona Rolla; Niccolò Rossi; Davide Brusa; Alessio Naccarati; Simon Couvreur; Tim D. Spector; Mario Roederer; Massimo Mangino; Francesca Cordero; Mario Falchi; Alessia Visconti

doi:10.3389/fimmu.2021.637974

Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women

Giulia Piaggeschi, Simona Rolla, Niccolò Rossi, Davide Brusa, Alessio Naccarati, Simon Couvreur, Tim D. Spector, Mario Roederer, Massimo Mangino, Francesca Cordero, Mario Falchi, Alessia Visconti

Twin Research & Genetic Epidemiology

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation.

Original language	English
Article number	637974
Journal	Frontiers in Immunology
Volume	12
DOIs	https://doi.org/10.3389/fimmu.2021.637974
Publication status	Published - 9 Mar 2021

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10.3389/fimmu.2021.637974

https://www.frontiersin.org/articles/10.3389/fimmu.2021.637974/full

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@article{d38d7a2a0e7b464ebb4cf445f8af510d,

title = "Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women",

abstract = "Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation.",

author = "Giulia Piaggeschi and Simona Rolla and Niccol{\`o} Rossi and Davide Brusa and Alessio Naccarati and Simon Couvreur and Spector, {Tim D.} and Mario Roederer and Massimo Mangino and Francesca Cordero and Mario Falchi and Alessia Visconti",

note = "Funding Information: TwinsUK was funded by the Wellcome Trust, Medical Research Council, European Union, Chronic Disease Research Foundation (CDRF), and the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility, and Biomedical Research Centre based at Guy{\textquoteright}s and St Thomas{\textquoteright} NHS Foundation Trust in partnership with King{\textquoteright}s College London. GP and AN were supported by the Italian Institute for Genomic Medicine (IIGM) and Compagnia di San Paolo, Torino, Italy. GP was supported by the ERASMUS+ traineeship fellowship. MR was supported by the intramural research program of the VRC, NAIAD, NIH. MM was supported by the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility and Biomedical Research Centre based at Guy{\textquoteright}s and St Thomas{\textquoteright} NHS Foundation Trust in partnership with King{\textquoteright}s College London. MF and AV were supported by funding from the Medical Research Council (MR/T004142/1). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Piaggeschi, Rolla, Rossi, Brusa, Naccarati, Couvreur, Spector, Roederer, Mangino, Cordero, Falchi and Visconti. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = mar,

day = "9",

doi = "10.3389/fimmu.2021.637974",

language = "English",

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journal = "Frontiers in Immunology",

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AU - Piaggeschi, Giulia

AU - Rolla, Simona

AU - Rossi, Niccolò

AU - Brusa, Davide

AU - Naccarati, Alessio

AU - Couvreur, Simon

AU - Spector, Tim D.

AU - Roederer, Mario

AU - Mangino, Massimo

AU - Cordero, Francesca

AU - Falchi, Mario

AU - Visconti, Alessia

N1 - Funding Information: TwinsUK was funded by the Wellcome Trust, Medical Research Council, European Union, Chronic Disease Research Foundation (CDRF), and the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility, and Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust in partnership with King’s College London. GP and AN were supported by the Italian Institute for Genomic Medicine (IIGM) and Compagnia di San Paolo, Torino, Italy. GP was supported by the ERASMUS+ traineeship fellowship. MR was supported by the intramural research program of the VRC, NAIAD, NIH. MM was supported by the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility and Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust in partnership with King’s College London. MF and AV were supported by funding from the Medical Research Council (MR/T004142/1). Publisher Copyright: © Copyright © 2021 Piaggeschi, Rolla, Rossi, Brusa, Naccarati, Couvreur, Spector, Roederer, Mangino, Cordero, Falchi and Visconti. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3/9

Y1 - 2021/3/9

N2 - Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation.

AB - Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation.

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U2 - 10.3389/fimmu.2021.637974

DO - 10.3389/fimmu.2021.637974

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SN - 1664-3224

VL - 12

JO - Frontiers in Immunology

JF - Frontiers in Immunology

M1 - 637974

ER -

Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women

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