King's College London

Funding Information: Funding This work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001002, FC001169, FC001745, FC001130), the UK Medical Research Council (FC001002, FC001169, FC001745, FC001130), and the Wellcome Trust (FC001002, FC001169, FC001745, FC001130). Francesca D. Ciccarelli is supported by Cancer Research UK (C43634/A25487), Guys and St Thomas Charity (R170504), the European Union’s Horizon 2020 Research and Innovation programme under the Marie Skłodowska-Curie grant agreement No. CONTRA-766030, the Cancer Research UK King’s Health Partners Centre at King’s College London (C604/A25135), and the Cancer Research UK City of London Centre (C7893/A26233). Funding Information: The authors thank Professor Rebecca Fitzgerald (University of Cambridge) and Professor Toby Lawrence (King’s College London) for critical comments to the manuscript. The authors acknowledge technical support from the Advanced Sequencing, the Flow Cytometry and the Experimental Histopathology Platforms of The Francis Crick Institute, the National Institute for Health Research Biomedical Research Centre Immune Monitoring Core Facility Centre based at Guy’s and St Thomas’ National Health Service Foundation Trust and King’s College London, Christopher Applebee and Sánchez-Magraner for the FRET analysis, and Joe Brock for graphical illustrations. The views expressed are those of the authors and not necessarily those of the National Health Service, the National Institute for Health Research or the United Kingdom Department of Health. The results published here are in part based upon data generated by The Cancer Genome Atlas managed by the National Cancer Institute and National Human Genome Research Institute. WES (EGAD00001006165) and RNA sequencing (EGAD00001006164) data have been deposited in the European Genome-phenome Archive. TCR-seq ( https://doi.org/10.5281/zenodo.3744371 ) and IMC ( https://doi.org/10.5281/zenodo.3743253 ) data have been deposited in Zenodo. The code used in this study and all supporting data are available upon request. Funding Information: The authors thank Professor Rebecca Fitzgerald (University of Cambridge) and Professor Toby Lawrence (King's College London) for critical comments to the manuscript. The authors acknowledge technical support from the Advanced Sequencing, the Flow Cytometry and the Experimental Histopathology Platforms of The Francis Crick Institute, the National Institute for Health Research Biomedical Research Centre Immune Monitoring Core Facility Centre based at Guy's and St Thomas? National Health Service Foundation Trust and King's College London, Christopher Applebee and S?nchez-Magraner for the FRET analysis, and Joe Brock for graphical illustrations. The views expressed are those of the authors and not necessarily those of the National Health Service, the National Institute for Health Research or the United Kingdom Department of Health. The results published here are in part based upon data generated by The Cancer Genome Atlas managed by the National Cancer Institute and National Human Genome Research Institute. WES (EGAD00001006165) and RNA sequencing (EGAD00001006164) data have been deposited in the European Genome-phenome Archive. TCR-seq (https://doi.org/10.5281/zenodo.3744371) and IMC (https://doi.org/10.5281/zenodo.3743253) data have been deposited in Zenodo. The code used in this study and all supporting data are available upon request. Michele Bertolomeazzi, MRes (Formal analysis: Equal; Software: Lead; Writing ? original draft: Equal). Reda Keddar, MRes (Formal analysis: Equal; Methodology: Lead; Writing ? original draft: Supporting). Lucia Montorsi, PhD (Data curation: Equal; Formal analysis: Equal; Writing ? original draft: Supporting). Amelia Acha, PhD (Data curation: Equal; Formal analysis: Equal; Writing ? original draft: Supporting). Lorena Benedetti, PhD (Data curation: Supporting; Formal analysis: Supporting). Damjan Temelkowski, PhD (Methodology: Supporting; Software: Supporting). Subin Choi, MRes (Data curation: Supporting). Nedyalko Petrov, MRes (Methodology: Supporting). Katrina Todd, PhD (Methodology: Supporting). Patty Way, PhD (Methodology: Supporting). Jonny Kohl, PhD (Methodology: Supporting). Tamara Denner, PhD (Methodology: Supporting). Emma Nye, PhD (Methodology: Supporting). Robert Goldstone, PhD (Methodology: Supporting). Sophia Ward, PhD (Methodology: Supporting). Gareth Wilson, PhD (Methodology: Supporting). Maise Al Bakir, PhD (Methodology: Supporting). Charles Swanton, PhD (Methodology: Supporting). Susan John, PhD (Formal analysis: Supporting). James Miles, MRes (Methodology: Supporting). Banafshe Larijani, PhD (Methodology: Supporting). Victoria Kunene, PhD (Resources: Supporting). Elisa Fontana, PhD (Resources: Supporting). Toby Arkenau, PhD (Resources: Supporting). Peter Parker, PhD (Methodology: Supporting). Manuel Rodriguez-Justo, PhD (Formal analysis: Supporting; Investigation: Supporting; Resources: Lead; Writing ? original draft: Supporting). Kai-Keen Shiu, MD PhD (Investigation: Supporting; Resources: Lead; Writing ? original draft: Supporting). Jo Spencer, PhD (Conceptualization: Equal; Formal analysis: Equal; Writing ? original draft: Equal). Francesca Ciccarelli, PhD (Conceptualization: Equal; Funding acquisition: Lead; Investigation: Lead; Supervision: Lead; Writing ? original draft: Equal). Funding This work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001002, FC001169, FC001745, FC001130), the UK Medical Research Council (FC001002, FC001169, FC001745, FC001130), and the Wellcome Trust (FC001002, FC001169, FC001745, FC001130). Francesca D. Ciccarelli is supported by Cancer Research UK (C43634/A25487), Guys and St Thomas Charity (R170504), the European Union's Horizon 2020 Research and Innovation programme under the Marie Sk?odowska-Curie grant agreement No. CONTRA-766030, the Cancer Research UK King's Health Partners Centre at King's College London (C604/A25135), and the Cancer Research UK City of London Centre (C7893/A26233). Publisher Copyright: © 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.