Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets

Hélio M. Gil; Zoe Booth; Thomas W. Price; Jessica Lee; Leigh Naylor-Adamson; Michelle Avery; Alina Muravitskaya; Nicole Hondow; David Allsup; Jürgen E. Schneider; Khalid Naseem; Ali M. Adawi; Jean Sebastien G. Bouillard; Thomas W. Chamberlain; Simon D.J. Calaminus; Graeme J. Stasiuk

doi:10.1002/smll.202304881

Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets

Hélio M. Gil, Zoe Booth, Thomas W. Price, Jessica Lee, Leigh Naylor-Adamson, Michelle Avery, Alina Muravitskaya, Nicole Hondow, David Allsup, Jürgen E. Schneider, Khalid Naseem, Ali M. Adawi, Jean Sebastien G. Bouillard, Thomas W. Chamberlain, Simon D.J. Calaminus^*, Graeme J. Stasiuk^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

InP/ZnS quantum dots (QDs) have received a large focus in recent years as a safer alternative to heavy metal-based QDs. Given their intrinsic fluorescent imaging capabilities, these QDs can be potentially relevant for in vivo platelet imaging. The InP/ZnS QDs are synthesized and their biocompatibility investigated through the use of different phase transfer agents. Analysis of platelet function indicates that platelet-QD interaction can occur at all concentrations and for all QD permutations tested. However, as the QD concentration increases, platelet aggregation is induced by QDs alone independent of natural platelet agonists. This study helps to define a range of concentrations and coatings (thioglycolic acid and penicillamine) that are biocompatible with platelet function. With this information, the platelet-QD interaction can be identified using multiple methods. Fluorescent lifetime imaging microscopy (FLIM) and confocal studies have shown QDs localize on the surface of the platelet toward the center while showing evidence of energy transfer within the QD population. It is believed that these findings are an important stepping point for the development of fluorescent probes for platelet imaging.

Original language	English
Article number	2304881
Journal	Small
Volume	20
Issue number	12
DOIs	https://doi.org/10.1002/smll.202304881
Publication status	Published - 22 Mar 2024

Keywords

fluorescence
fluorescence lifetime imaging microscopy (FLIM)
hot-injections
InP/ZnS quantum dots (QDs)
platelet imaging
platelets biocompatibility

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10.1002/smll.202304881

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Gil, H. M., Booth, Z., Price, T. W., Lee, J., Naylor-Adamson, L., Avery, M., Muravitskaya, A., Hondow, N., Allsup, D., Schneider, J. E., Naseem, K., Adawi, A. M., Bouillard, J. S. G., Chamberlain, T. W., Calaminus, S. D. J., & Stasiuk, G. J. (2024). Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets. Small, 20(12), Article 2304881. https://doi.org/10.1002/smll.202304881

@article{bbe45f3622ea4ceea112232e61bd5f25,

title = "Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets",

abstract = "InP/ZnS quantum dots (QDs) have received a large focus in recent years as a safer alternative to heavy metal-based QDs. Given their intrinsic fluorescent imaging capabilities, these QDs can be potentially relevant for in vivo platelet imaging. The InP/ZnS QDs are synthesized and their biocompatibility investigated through the use of different phase transfer agents. Analysis of platelet function indicates that platelet-QD interaction can occur at all concentrations and for all QD permutations tested. However, as the QD concentration increases, platelet aggregation is induced by QDs alone independent of natural platelet agonists. This study helps to define a range of concentrations and coatings (thioglycolic acid and penicillamine) that are biocompatible with platelet function. With this information, the platelet-QD interaction can be identified using multiple methods. Fluorescent lifetime imaging microscopy (FLIM) and confocal studies have shown QDs localize on the surface of the platelet toward the center while showing evidence of energy transfer within the QD population. It is believed that these findings are an important stepping point for the development of fluorescent probes for platelet imaging.",

keywords = "fluorescence, fluorescence lifetime imaging microscopy (FLIM), hot-injections, InP/ZnS quantum dots (QDs), platelet imaging, platelets biocompatibility",

author = "Gil, {H{\'e}lio M.} and Zoe Booth and Price, {Thomas W.} and Jessica Lee and Leigh Naylor-Adamson and Michelle Avery and Alina Muravitskaya and Nicole Hondow and David Allsup and Schneider, {J{\"u}rgen E.} and Khalid Naseem and Adawi, {Ali M.} and Bouillard, {Jean Sebastien G.} and Chamberlain, {Thomas W.} and Calaminus, {Simon D.J.} and Stasiuk, {Graeme J.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors. Small published by Wiley-VCH GmbH.",

year = "2024",

month = mar,

day = "22",

doi = "10.1002/smll.202304881",

language = "English",

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Gil, HM, Booth, Z, Price, TW, Lee, J, Naylor-Adamson, L, Avery, M, Muravitskaya, A, Hondow, N, Allsup, D, Schneider, JE, Naseem, K, Adawi, AM, Bouillard, JSG, Chamberlain, TW, Calaminus, SDJ & Stasiuk, GJ 2024, 'Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets', Small, vol. 20, no. 12, 2304881. https://doi.org/10.1002/smll.202304881

TY - JOUR

T1 - Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets

AU - Gil, Hélio M.

AU - Booth, Zoe

AU - Price, Thomas W.

AU - Lee, Jessica

AU - Naylor-Adamson, Leigh

AU - Avery, Michelle

AU - Muravitskaya, Alina

AU - Hondow, Nicole

AU - Allsup, David

AU - Schneider, Jürgen E.

AU - Naseem, Khalid

AU - Adawi, Ali M.

AU - Bouillard, Jean Sebastien G.

AU - Chamberlain, Thomas W.

AU - Calaminus, Simon D.J.

AU - Stasiuk, Graeme J.

PY - 2024/3/22

Y1 - 2024/3/22

N2 - InP/ZnS quantum dots (QDs) have received a large focus in recent years as a safer alternative to heavy metal-based QDs. Given their intrinsic fluorescent imaging capabilities, these QDs can be potentially relevant for in vivo platelet imaging. The InP/ZnS QDs are synthesized and their biocompatibility investigated through the use of different phase transfer agents. Analysis of platelet function indicates that platelet-QD interaction can occur at all concentrations and for all QD permutations tested. However, as the QD concentration increases, platelet aggregation is induced by QDs alone independent of natural platelet agonists. This study helps to define a range of concentrations and coatings (thioglycolic acid and penicillamine) that are biocompatible with platelet function. With this information, the platelet-QD interaction can be identified using multiple methods. Fluorescent lifetime imaging microscopy (FLIM) and confocal studies have shown QDs localize on the surface of the platelet toward the center while showing evidence of energy transfer within the QD population. It is believed that these findings are an important stepping point for the development of fluorescent probes for platelet imaging.

AB - InP/ZnS quantum dots (QDs) have received a large focus in recent years as a safer alternative to heavy metal-based QDs. Given their intrinsic fluorescent imaging capabilities, these QDs can be potentially relevant for in vivo platelet imaging. The InP/ZnS QDs are synthesized and their biocompatibility investigated through the use of different phase transfer agents. Analysis of platelet function indicates that platelet-QD interaction can occur at all concentrations and for all QD permutations tested. However, as the QD concentration increases, platelet aggregation is induced by QDs alone independent of natural platelet agonists. This study helps to define a range of concentrations and coatings (thioglycolic acid and penicillamine) that are biocompatible with platelet function. With this information, the platelet-QD interaction can be identified using multiple methods. Fluorescent lifetime imaging microscopy (FLIM) and confocal studies have shown QDs localize on the surface of the platelet toward the center while showing evidence of energy transfer within the QD population. It is believed that these findings are an important stepping point for the development of fluorescent probes for platelet imaging.

KW - fluorescence

KW - fluorescence lifetime imaging microscopy (FLIM)

KW - hot-injections

KW - InP/ZnS quantum dots (QDs)

KW - platelet imaging

KW - platelets biocompatibility

UR - http://www.scopus.com/inward/record.url?scp=85176143462&partnerID=8YFLogxK

U2 - 10.1002/smll.202304881

DO - 10.1002/smll.202304881

M3 - Article

C2 - 37946631

AN - SCOPUS:85176143462

SN - 1613-6810

VL - 20

JO - Small

JF - Small

IS - 12

M1 - 2304881

ER -

Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets

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Keywords

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