In vitro and in vivo comparison of two-, three- and four-point Dixon techniques for clinical intramuscular fat quantification at 3T

J. J. Noble*, S. F. Keevil, J. Totman, G. D. Charles-Edwards

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Objective: To compare Dixon-based MRI techniques for intramuscular fat quantification at 3 T with MR spectroscopy (MRS) in vitro and in vivo.

Methods: In vitro, two-three-and four-point mDixon (Philips Medical Systems, Best, Netherlands) sequences with 10 degrees, 20 degrees and 30 degrees flip angles were acquired from seven test phantoms with sunflower oil-water percentages of 0-60% sunflower oil and calculated fat-water ratios compared with MRS. In vivo, two-three-and four-point mDixon sequences with 10 degrees flip angle were acquired and compared with MRS in the vastus medialis of nine healthy volunteers (aged 30.6 +/- 5.3 years; body mass index 22.2 +/- 2.6).

Results: In vitro, all mDixon sequences correlated significantly with MRS (r > 0.97, p <0.002). The measured phantom percentage fat depended significantly on the flip angle (p

Conclusion: This study demonstrates that mDixon techniques have good linearity and low variability for use in intramuscular fat quantification. To avoid significant fat overestimation with short repetition time, a low flip angle should be used to reduce T-1 effects.

Advances in knowledge: This is the first study investigating the optimal mDixon parameters for intramuscular fat quantification compared with MRS in vivo and in vitro.

Original languageEnglish
Number of pages7
JournalBritish Journal of Radiology
Volume87
Issue number1036
DOIs
Publication statusPublished - Apr 2014

Keywords

  • DUCHENNE MUSCULAR-DYSTROPHY
  • ENERGY-EXPENDITURE
  • BODY-COMPOSITION
  • CEREBRAL-PALSY
  • ADIPOSE-TISSUE
  • MUSCLE
  • WATER
  • SEPARATION
  • PROTON
  • IDEAL

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