In vitro and in vivo comparison of two-, three- and four-point Dixon techniques for clinical intramuscular fat quantification at 3T

TY - JOUR

T1 - In vitro and in vivo comparison of two-, three- and four-point Dixon techniques for clinical intramuscular fat quantification at 3T

AU - Noble, J. J.

AU - Keevil, S. F.

AU - Totman, J.

AU - Charles-Edwards, G. D.

PY - 2014/4

Y1 - 2014/4

N2 - Objective: To compare Dixon-based MRI techniques for intramuscular fat quantification at 3 T with MR spectroscopy (MRS) in vitro and in vivo.Methods: In vitro, two-three-and four-point mDixon (Philips Medical Systems, Best, Netherlands) sequences with 10 degrees, 20 degrees and 30 degrees flip angles were acquired from seven test phantoms with sunflower oil-water percentages of 0-60% sunflower oil and calculated fat-water ratios compared with MRS. In vivo, two-three-and four-point mDixon sequences with 10 degrees flip angle were acquired and compared with MRS in the vastus medialis of nine healthy volunteers (aged 30.6 +/- 5.3 years; body mass index 22.2 +/- 2.6).Results: In vitro, all mDixon sequences correlated significantly with MRS (r > 0.97, p <0.002). The measured phantom percentage fat depended significantly on the flip angle (p Conclusion: This study demonstrates that mDixon techniques have good linearity and low variability for use in intramuscular fat quantification. To avoid significant fat overestimation with short repetition time, a low flip angle should be used to reduce T-1 effects.Advances in knowledge: This is the first study investigating the optimal mDixon parameters for intramuscular fat quantification compared with MRS in vivo and in vitro.

AB - Objective: To compare Dixon-based MRI techniques for intramuscular fat quantification at 3 T with MR spectroscopy (MRS) in vitro and in vivo.Methods: In vitro, two-three-and four-point mDixon (Philips Medical Systems, Best, Netherlands) sequences with 10 degrees, 20 degrees and 30 degrees flip angles were acquired from seven test phantoms with sunflower oil-water percentages of 0-60% sunflower oil and calculated fat-water ratios compared with MRS. In vivo, two-three-and four-point mDixon sequences with 10 degrees flip angle were acquired and compared with MRS in the vastus medialis of nine healthy volunteers (aged 30.6 +/- 5.3 years; body mass index 22.2 +/- 2.6).Results: In vitro, all mDixon sequences correlated significantly with MRS (r > 0.97, p <0.002). The measured phantom percentage fat depended significantly on the flip angle (p Conclusion: This study demonstrates that mDixon techniques have good linearity and low variability for use in intramuscular fat quantification. To avoid significant fat overestimation with short repetition time, a low flip angle should be used to reduce T-1 effects.Advances in knowledge: This is the first study investigating the optimal mDixon parameters for intramuscular fat quantification compared with MRS in vivo and in vitro.

KW - DUCHENNE MUSCULAR-DYSTROPHY

KW - ENERGY-EXPENDITURE

KW - BODY-COMPOSITION

KW - CEREBRAL-PALSY

KW - ADIPOSE-TISSUE

KW - MUSCLE

KW - WATER

KW - SEPARATION

KW - PROTON

KW - IDEAL

U2 - 10.1259/bjr.20130761

DO - 10.1259/bjr.20130761

M3 - Article

SN - 0007-1285

VL - 87

JO - British Journal of Radiology

JF - British Journal of Radiology

IS - 1036

ER -