In vitro cytotoxicity of norviburtinal and isopinnatal from Kigelia pinnata against cancer cell lines

S J Jackson; P J Houghton; S Retsas; A Photiou

In vitro cytotoxicity of norviburtinal and isopinnatal from Kigelia pinnata against cancer cell lines

S J Jackson, P J Houghton, S Retsas, A Photiou

Research output: Contribution to journal › Letter › peer-review

56 Citations (Scopus)

Abstract

Crude dichloromethane extracts of Kigelia pinnata stem bark and fruit showed cytotoxic activity in vitro against cultured melanoma and other cancer cell lines using the Sulphorhodamine B assay, which was used for bioassay-guided fractionation. Thin layer chromatography (TLC) examination of the most active fractions of both stem bark and fruits showed the presence of the same major components which were found to be norviburtinal and beta -sitosterol. Norviburtinal was found to be the most active compound but had little selectivity for melanoma cell lines whilst isopinnatal also showed some cytotoxic: activity. beta -Sitosterol was found to be comparatively inactive. HPLC analysis of the crude extract showed that the amount of norviburtinal present in the plant material did not account for all of the activity of the total extracts.

Original language	English
Pages (from-to)	758 - 761
Number of pages	4
Journal	Planta Medica
Volume	66
Issue number	8
Publication status	Published - 2000

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title = "In vitro cytotoxicity of norviburtinal and isopinnatal from Kigelia pinnata against cancer cell lines",

abstract = "Crude dichloromethane extracts of Kigelia pinnata stem bark and fruit showed cytotoxic activity in vitro against cultured melanoma and other cancer cell lines using the Sulphorhodamine B assay, which was used for bioassay-guided fractionation. Thin layer chromatography (TLC) examination of the most active fractions of both stem bark and fruits showed the presence of the same major components which were found to be norviburtinal and beta -sitosterol. Norviburtinal was found to be the most active compound but had little selectivity for melanoma cell lines whilst isopinnatal also showed some cytotoxic: activity. beta -Sitosterol was found to be comparatively inactive. HPLC analysis of the crude extract showed that the amount of norviburtinal present in the plant material did not account for all of the activity of the total extracts.",

author = "Jackson, {S J} and Houghton, {P J} and S Retsas and A Photiou",

year = "2000",

language = "English",

volume = "66",

pages = "758 -- 761",

journal = "Planta Medica",

publisher = "Georg Thieme Verlag",

number = "8",

}

TY - JOUR

T1 - In vitro cytotoxicity of norviburtinal and isopinnatal from Kigelia pinnata against cancer cell lines

AU - Jackson, S J

AU - Houghton, P J

AU - Retsas, S

AU - Photiou, A

PY - 2000

Y1 - 2000

N2 - Crude dichloromethane extracts of Kigelia pinnata stem bark and fruit showed cytotoxic activity in vitro against cultured melanoma and other cancer cell lines using the Sulphorhodamine B assay, which was used for bioassay-guided fractionation. Thin layer chromatography (TLC) examination of the most active fractions of both stem bark and fruits showed the presence of the same major components which were found to be norviburtinal and beta -sitosterol. Norviburtinal was found to be the most active compound but had little selectivity for melanoma cell lines whilst isopinnatal also showed some cytotoxic: activity. beta -Sitosterol was found to be comparatively inactive. HPLC analysis of the crude extract showed that the amount of norviburtinal present in the plant material did not account for all of the activity of the total extracts.

AB - Crude dichloromethane extracts of Kigelia pinnata stem bark and fruit showed cytotoxic activity in vitro against cultured melanoma and other cancer cell lines using the Sulphorhodamine B assay, which was used for bioassay-guided fractionation. Thin layer chromatography (TLC) examination of the most active fractions of both stem bark and fruits showed the presence of the same major components which were found to be norviburtinal and beta -sitosterol. Norviburtinal was found to be the most active compound but had little selectivity for melanoma cell lines whilst isopinnatal also showed some cytotoxic: activity. beta -Sitosterol was found to be comparatively inactive. HPLC analysis of the crude extract showed that the amount of norviburtinal present in the plant material did not account for all of the activity of the total extracts.

M3 - Letter

VL - 66

SP - 758

EP - 761

JO - Planta Medica

JF - Planta Medica

IS - 8

ER -

In vitro cytotoxicity of norviburtinal and isopinnatal from Kigelia pinnata against cancer cell lines

Abstract

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