In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers

TY - JOUR

T1 - In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers

AU - Leptihn, Sebastian

AU - Thompson, James R.

AU - Ellory, J. Clive

AU - Tucker, Stephen J.

AU - Wallace, Mark I.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.

AB - The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.

U2 - 10.1021/ja200128n

DO - 10.1021/ja200128n

M3 - Article

VL - 133

SP - 9370

EP - 9375

JO - J. Am. Chem. Soc.

JF - J. Am. Chem. Soc.

IS - 24

ER -