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In Vivo Acoustic Super-Resolution and Super-Resolved Velocity Mapping Using Microbubbles

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Pages (from-to)433-440
JournalIeee Transactions on Medical Imaging
Issue number2
Early online date23 Sep 2014
Accepted/In press4 Sep 2014
E-pub ahead of print23 Sep 2014
Published28 Feb 2015


  • In Vivo Acoustic Super_CHRISTENSEN-JEFFRIES_Published Online 23 September 2014_Green AAM

    In_Vivo_Acoustic_Super_Resolution_and_Super_Resolved_Velocity_Mapping_Using_Microbubbles.pdf, 8.04 MB, application/pdf

    Uploaded date:10 Oct 2014

    Version:Accepted author manuscript

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King's Authors


The structure of microvasculature cannot be resolved using standard clinical ultrasound (US) imaging frequencies due to the fundamental diffraction limit of US waves. In this work, we use a standard clinical US system to perform in vivo sub-diffraction imaging on a CD1, female mouse aged 8 weeks by localizing isolated US signals from bubbles flowing within the ear microvasculature, and compare our results to optical microscopy. Furthermore, we develop a new technique to map blood velocity at super-resolution by tracking individual bubbles through the vasculature. Resolution is improved from a measured lateral and axial resolution of 112 μm and 94 μm respectively in original US data, to super-resolved images of microvasculature where vessel features as fine as 19 μm are clearly visualized. Velocity maps clearly distinguish opposing flow direction and separated speed distributions in adjacent vessels, thereby enabling further differentiation between vessels otherwise not spatially separated in the image. This technique overcomes the diffraction limit to provide a non-invasive means of imaging the microvasculature at super-resolution, to depths of many centimeters. In the future, this method could noninvasively image pathological or therapeutic changes in the microvasculature at centimeter depths in vivo.

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