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In Vivo Acoustic Super-Resolution and Super-Resolved Velocity Mapping Using Microbubbles

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)433-440
JournalIeee Transactions on Medical Imaging
Volume34
Issue number2
Early online date23 Sep 2014
DOIs
Publication statusPublished - 23 Sep 2014

Documents

  • In Vivo Acoustic Super_CHRISTENSEN-JEFFRIES_Published Online 23 September 2014_Green AAM

    In_Vivo_Acoustic_Super_Resolution_and_Super_Resolved_Velocity_Mapping_Using_Microbubbles.pdf, 8.04 MB, application/pdf

    10/10/2014

    Accepted author manuscript

    (c) 2013 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.

King's Authors

Abstract

The structure of microvasculature cannot be resolved using standard clinical ultrasound (US) imaging frequencies due to the fundamental diffraction limit of US waves. In this work, we use a standard clinical US system to perform in vivo sub-diffraction imaging on a CD1, female mouse aged 8 weeks by localizing isolated US signals from bubbles flowing within the ear microvasculature, and compare our results to optical microscopy. Furthermore, we develop a new technique to map blood velocity at super-resolution by tracking individual bubbles through the vasculature. Resolution is improved from a measured lateral and axial resolution of 112 μm and 94 μm respectively in original US data, to super-resolved images of microvasculature where vessel features as fine as 19 μm are clearly visualized. Velocity maps clearly distinguish opposing flow direction and separated speed distributions in adjacent vessels, thereby enabling further differentiation between vessels otherwise not spatially separated in the image. This technique overcomes the diffraction limit to provide a non-invasive means of imaging the microvasculature at super-resolution, to depths of many centimeters. In the future, this method could noninvasively image pathological or therapeutic changes in the microvasculature at centimeter depths in vivo.

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