In vivo and in vitro knockdown approaches in the avian embryo as a means to study semaphorin signaling

Karli Montague, Sarah Guthrie*, Ivana Poparic

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A combination of both in vivo and in vitro techniques is invaluable for studying semaphorin signaling in the avian central nervous system. Here we describe how both types of approaches can be used to compliment each other in order to unravel the role that semaphorins play during embryonic development and elucidate the functional consequences of semaphorin knockdown using RNA interference vectors. We describe and discuss specifically the use of in ovo electroporation and primary oculomotor neuron culture to identify the role of semaphorins in oculomotor neuron migration and assess functional consequences of semaphorin disruption in this system.

Original languageEnglish
Pages (from-to)403-416
Number of pages14
JournalMethods in Molecular Biology
Volume1493
DOIs
Publication statusPublished - 2017

Keywords

  • Avian
  • In ovo electroporation
  • In vitro
  • In vivo
  • Oculomotor culture
  • Semaphorin
  • Transf

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