TY - JOUR
T1 - In vivo calcium imaging shows that satellite glial cells have increased activity in painful states
AU - Jager, Sara E
AU - Goodwin, George
AU - Chisholm, Kim I
AU - Denk, Franziska
N1 - Funding Information:
This research was supported by a Lundbeck Foundation Fellowship (R293-2018-960) and a grant from IMK Almene Fond (30206-372) to S.E.J. F.D. has received funding through a Medical Research Council New Investigator Research Grant (MR/P010814/1) and a Medical Research Foundation Prize (MRF-160-0015-ELP-DENK-C0844). G.G. is funded by an Advanced Pain Discovery Platform grant from United Kingdom Research and Innovation Medical Research Council (MR/W027518/1). K.I.C. is funded by the University of Nottingham via an Anne McLaren Fellowship. The research has also received support from the National Institute for Health and Care Research (NIHR) Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust and King’s College London. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health and Social Care. This research was funded in whole or in part by the UK Research and Innovation.
Publisher Copyright:
© The Author(s) 2024.
PY - 2024
Y1 - 2024
N2 - Satellite glial cells are important for proper neuronal function of primary sensory neurons for which they provide homeostatic support. Most research on satellite glial cell function has been performed with in vitro studies, but recent advances in calcium imaging and transgenic mouse models have enabled this first in vivo study of single-cell satellite glial cell function in mouse models of inflammation and neuropathic pain. We found that in naïve conditions, satellite glial cells do not respond in a time-locked fashion to neuronal firing. In painful inflammatory and neuropathic states, we detected time-locked signals in a subset of satellite glial cells, but only with suprathreshold stimulation of the sciatic nerve. Surprisingly, therefore, we conclude that most calcium signals in satellite glial cells seem to develop at arbitrary intervals not directly linked to neuronal activity patterns. More in line with expectations, our experiments also revealed that the number of active satellite glial cells was increased under conditions of inflammation or nerve injury. This could reflect the increased requirement for homeostatic support across dorsal root ganglion neuron populations, which are more active during such painful states.
AB - Satellite glial cells are important for proper neuronal function of primary sensory neurons for which they provide homeostatic support. Most research on satellite glial cell function has been performed with in vitro studies, but recent advances in calcium imaging and transgenic mouse models have enabled this first in vivo study of single-cell satellite glial cell function in mouse models of inflammation and neuropathic pain. We found that in naïve conditions, satellite glial cells do not respond in a time-locked fashion to neuronal firing. In painful inflammatory and neuropathic states, we detected time-locked signals in a subset of satellite glial cells, but only with suprathreshold stimulation of the sciatic nerve. Surprisingly, therefore, we conclude that most calcium signals in satellite glial cells seem to develop at arbitrary intervals not directly linked to neuronal activity patterns. More in line with expectations, our experiments also revealed that the number of active satellite glial cells was increased under conditions of inflammation or nerve injury. This could reflect the increased requirement for homeostatic support across dorsal root ganglion neuron populations, which are more active during such painful states.
UR - http://www.scopus.com/inward/record.url?scp=85187531770&partnerID=8YFLogxK
U2 - 10.1093/braincomms/fcae013
DO - 10.1093/braincomms/fcae013
M3 - Article
C2 - 38638153
SN - 2632-1297
VL - 6
JO - Brain Communications
JF - Brain Communications
IS - 2
M1 - fcae013
ER -