In Vivo High-Frequency Ultrasound for the Characterization of Thrombi Associated with Aortic Aneurysms in an Experimental Mouse Model

Christian H.P. Jansen; Julia Brangsch; Carolin Reimann; Lisa Adams; Bernd Hamm; Rene M. Botnar; Marcus R. Makowski

doi:10.1016/j.ultrasmedbio.2017.08.007

In Vivo High-Frequency Ultrasound for the Characterization of Thrombi Associated with Aortic Aneurysms in an Experimental Mouse Model

Christian H.P. Jansen, Julia Brangsch, Carolin Reimann, Lisa Adams, Bernd Hamm, Rene M. Botnar, Marcus R. Makowski

Biomedical Engineering Department

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Abstract

The development of abdominal aortic aneurysm (AAA) associated thrombi plays an important role during the onset and progression of AAAs. The aim of this study was to evaluate the potential of high-frequency ultrasound for characterization of AAA associated thrombi in an apolipoprotein-E-deficient mouse-model. Ultrasound measurements were performed using a high-resolution ultrasound system (Vevo770, FUJIFILM VisualSonics, Inc., Toronto, ON, Canada) with a 30 MHz linear-array transducer (RMV707 B). Magnetic resonance imaging with a 3 Tesla scanner (Achieva MR system, Philips Healthcare, Best, The Netherlands) and a single-loop microscopy coil was performed as a reference standard. All stages of aneurysm development were evaluated by histologic analyses. The “signal-thrombus-matrix” to “signal-blood” ratio on high-frequency ultrasound measurements showed a strong correlation (R2 = 0.81, p < 0.05) with the state of extracellular matrix remodeling. Furthermore, size measurements derived from the high-frequency ultrasound correlated well with magnetic resonance imaging and histology. This study demonstrated that high-frequency ultrasound enables the reliable in vivo quantification of extracellular matrix remodeling at various stages of thrombus development, based on the thrombus echogenicity.

Original language	English
Journal	Ultrasound in Medicine and Biology
Early online date	28 Sept 2017
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2017.08.007
Publication status	E-pub ahead of print - 28 Sept 2017

Keywords

Abdominal aortic aneurysms
Ultrasound
Magnetic resonance imaging

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10.1016/j.ultrasmedbio.2017.08.007

In Vivo High-Frequency Ultrasound_JANSEN_Publishedonline28September2017_GREEN AAM (CC BY-NC-ND)Accepted author manuscript, 224 KBLicence: CC BY-NC-ND

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title = "In Vivo High-Frequency Ultrasound for the Characterization of Thrombi Associated with Aortic Aneurysms in an Experimental Mouse Model",

abstract = "The development of abdominal aortic aneurysm (AAA) associated thrombi plays an important role during the onset and progression of AAAs. The aim of this study was to evaluate the potential of high-frequency ultrasound for characterization of AAA associated thrombi in an apolipoprotein-E-deficient mouse-model. Ultrasound measurements were performed using a high-resolution ultrasound system (Vevo770, FUJIFILM VisualSonics, Inc., Toronto, ON, Canada) with a 30 MHz linear-array transducer (RMV707 B). Magnetic resonance imaging with a 3 Tesla scanner (Achieva MR system, Philips Healthcare, Best, The Netherlands) and a single-loop microscopy coil was performed as a reference standard. All stages of aneurysm development were evaluated by histologic analyses. The “signal-thrombus-matrix” to “signal-blood” ratio on high-frequency ultrasound measurements showed a strong correlation (R2 = 0.81, p < 0.05) with the state of extracellular matrix remodeling. Furthermore, size measurements derived from the high-frequency ultrasound correlated well with magnetic resonance imaging and histology. This study demonstrated that high-frequency ultrasound enables the reliable in vivo quantification of extracellular matrix remodeling at various stages of thrombus development, based on the thrombus echogenicity.",

keywords = "Abdominal aortic aneurysms, Ultrasound, Magnetic resonance imaging",

author = "Jansen, {Christian H.P.} and Julia Brangsch and Carolin Reimann and Lisa Adams and Bernd Hamm and Botnar, {Rene M.} and Makowski, {Marcus R.}",

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doi = "10.1016/j.ultrasmedbio.2017.08.007",

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AU - Jansen, Christian H.P.

AU - Brangsch, Julia

AU - Reimann, Carolin

AU - Adams, Lisa

AU - Hamm, Bernd

AU - Botnar, Rene M.

AU - Makowski, Marcus R.

PY - 2017/9/28

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N2 - The development of abdominal aortic aneurysm (AAA) associated thrombi plays an important role during the onset and progression of AAAs. The aim of this study was to evaluate the potential of high-frequency ultrasound for characterization of AAA associated thrombi in an apolipoprotein-E-deficient mouse-model. Ultrasound measurements were performed using a high-resolution ultrasound system (Vevo770, FUJIFILM VisualSonics, Inc., Toronto, ON, Canada) with a 30 MHz linear-array transducer (RMV707 B). Magnetic resonance imaging with a 3 Tesla scanner (Achieva MR system, Philips Healthcare, Best, The Netherlands) and a single-loop microscopy coil was performed as a reference standard. All stages of aneurysm development were evaluated by histologic analyses. The “signal-thrombus-matrix” to “signal-blood” ratio on high-frequency ultrasound measurements showed a strong correlation (R2 = 0.81, p < 0.05) with the state of extracellular matrix remodeling. Furthermore, size measurements derived from the high-frequency ultrasound correlated well with magnetic resonance imaging and histology. This study demonstrated that high-frequency ultrasound enables the reliable in vivo quantification of extracellular matrix remodeling at various stages of thrombus development, based on the thrombus echogenicity.

AB - The development of abdominal aortic aneurysm (AAA) associated thrombi plays an important role during the onset and progression of AAAs. The aim of this study was to evaluate the potential of high-frequency ultrasound for characterization of AAA associated thrombi in an apolipoprotein-E-deficient mouse-model. Ultrasound measurements were performed using a high-resolution ultrasound system (Vevo770, FUJIFILM VisualSonics, Inc., Toronto, ON, Canada) with a 30 MHz linear-array transducer (RMV707 B). Magnetic resonance imaging with a 3 Tesla scanner (Achieva MR system, Philips Healthcare, Best, The Netherlands) and a single-loop microscopy coil was performed as a reference standard. All stages of aneurysm development were evaluated by histologic analyses. The “signal-thrombus-matrix” to “signal-blood” ratio on high-frequency ultrasound measurements showed a strong correlation (R2 = 0.81, p < 0.05) with the state of extracellular matrix remodeling. Furthermore, size measurements derived from the high-frequency ultrasound correlated well with magnetic resonance imaging and histology. This study demonstrated that high-frequency ultrasound enables the reliable in vivo quantification of extracellular matrix remodeling at various stages of thrombus development, based on the thrombus echogenicity.

KW - Abdominal aortic aneurysms

KW - Ultrasound

KW - Magnetic resonance imaging

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DO - 10.1016/j.ultrasmedbio.2017.08.007

M3 - Article

SN - 0301-5629

JO - Ultrasound in Medicine and Biology

JF - Ultrasound in Medicine and Biology

ER -

In Vivo High-Frequency Ultrasound for the Characterization of Thrombi Associated with Aortic Aneurysms in an Experimental Mouse Model

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Keywords

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