TY - JOUR
T1 - In Vivo Magnetization Transfer and Diffusion Weighted MRI Detects Thrombus Composition in a Mouse Model of Deep Vein Thrombosis
AU - Phinikaridou, Alkystis
AU - Andia, Marcelo E
AU - Saha, Prakash
AU - Modarai, Bijan
AU - Smith, Alberto
AU - Botnar, René M
PY - 2013/4/5
Y1 - 2013/4/5
N2 - BACKGROUND: -Deep vein thrombosis (DVT) remains a major health problem necessitating accurate diagnosis. Thrombolysis is associated with significant morbidity and is only effective for treatment of unorganized thrombus. We tested the feasibility of in vivo magnetization transfer (MT) and diffusion weighted (DW) magnetic resonance imaging (MRI) to detect thrombus organization in a murine model of DVT. METHODS AND RESULTS: -DVT was induced in the inferior vena cava of male BALB/C mice. MRI was performed at day 1, 7, 14, 21, and 28 after thrombus induction using MT, DW, inversion recovery, and T1 mapping protocols. Delayed-enhancement and T1 mapping were repeated 2hr post-injection of a fibrin contrast agent. Finally, excised thrombi were used for histology. We found that MT and DW imaging can detect histological changes associated with thrombus aging. Magnetization transfer rate (MTR) maps and %MTR allowed visualization and quantification of the thrombus protein content, respectively. The %MTR increased with thrombus organization and was significantly higher at day 14, 21, and 28 after thrombus induction [day 1, 7, 14, 21, 28: %MTR =2483±451, 2079±1210, 7029±2490, 10295±4356, 32994±25449, Panova
AB - BACKGROUND: -Deep vein thrombosis (DVT) remains a major health problem necessitating accurate diagnosis. Thrombolysis is associated with significant morbidity and is only effective for treatment of unorganized thrombus. We tested the feasibility of in vivo magnetization transfer (MT) and diffusion weighted (DW) magnetic resonance imaging (MRI) to detect thrombus organization in a murine model of DVT. METHODS AND RESULTS: -DVT was induced in the inferior vena cava of male BALB/C mice. MRI was performed at day 1, 7, 14, 21, and 28 after thrombus induction using MT, DW, inversion recovery, and T1 mapping protocols. Delayed-enhancement and T1 mapping were repeated 2hr post-injection of a fibrin contrast agent. Finally, excised thrombi were used for histology. We found that MT and DW imaging can detect histological changes associated with thrombus aging. Magnetization transfer rate (MTR) maps and %MTR allowed visualization and quantification of the thrombus protein content, respectively. The %MTR increased with thrombus organization and was significantly higher at day 14, 21, and 28 after thrombus induction [day 1, 7, 14, 21, 28: %MTR =2483±451, 2079±1210, 7029±2490, 10295±4356, 32994±25449, Panova
U2 - 10.1161/CIRCIMAGING.112.000077
DO - 10.1161/CIRCIMAGING.112.000077
M3 - Article
C2 - 23564561
SN - 1942-0080
JO - Circulation-Cardiovascular imaging
JF - Circulation-Cardiovascular imaging
ER -
