TY - JOUR
T1 - In vivo study of the bioavailability and metabolic profile of (poly)phenols after sous-vide artichoke consumption
AU - Dominguez-Fernandez, Maite
AU - Young Tie Yang, Paul
AU - Ludwig, Iziar
AU - Clifford, Michael N
AU - Cid, Concepcion
AU - Rodriguez Mateos, Ana Maria
PY - 2021/7/16
Y1 - 2021/7/16
N2 - Artichokes are a rich source of (poly)phenols, mainly caffeoylquinic acids, but little is known about their bioavailability from this source. This study investigated the absorption, metabolism and excretion of (poly)phenols after sous-vide artichoke consumption (5776 µmol of (poly)phenols) by healthy volunteers. Seventy-six (poly)phenol metabolites were identified by UHPLC-MS/MS using authentic standards, including acyl-quinic acids plus C6–C3, C6–C1, C6–C2, C6–C2–N, C6–C0 metabolites, and their phase-II conjugates. The major metabolites were 3ʹ-methoxy-4ʹ-hydroxycinnamic acid, 3ʹ-methoxycinnamic acid-4ʹ-sulfate, and 4ʹ-hydroxycinnamic acid-3ʹ-sulfate, which appeared early in plasma (Tmax < 4 h); plus 3-(3ʹ-methoxy-4ʹ-hydroxyphenyl)propanoic acid, 3-(4ʹ-methoxyphenyl)propanoic acid-3ʹ-glucuronide, 3-(3ʹ-hydroxyphenyl) propanoic acid and hippuric acids, which appeared later (Tmax >6 h). The 24 h urinary recovery averaged 8.9% (molar basis) of the (poly)phenols consumed. Hepatic beta-oxidation of 3ʹ,4ʹ-dihydroxycinnamic acid and methylated conjugates occurred, but was limited (<0.04%). 3ʹ-Methylation exceeded 4ʹ-methylation and interindividual variability was high, especially for gut microbial metabolites (up to 168-fold).
AB - Artichokes are a rich source of (poly)phenols, mainly caffeoylquinic acids, but little is known about their bioavailability from this source. This study investigated the absorption, metabolism and excretion of (poly)phenols after sous-vide artichoke consumption (5776 µmol of (poly)phenols) by healthy volunteers. Seventy-six (poly)phenol metabolites were identified by UHPLC-MS/MS using authentic standards, including acyl-quinic acids plus C6–C3, C6–C1, C6–C2, C6–C2–N, C6–C0 metabolites, and their phase-II conjugates. The major metabolites were 3ʹ-methoxy-4ʹ-hydroxycinnamic acid, 3ʹ-methoxycinnamic acid-4ʹ-sulfate, and 4ʹ-hydroxycinnamic acid-3ʹ-sulfate, which appeared early in plasma (Tmax < 4 h); plus 3-(3ʹ-methoxy-4ʹ-hydroxyphenyl)propanoic acid, 3-(4ʹ-methoxyphenyl)propanoic acid-3ʹ-glucuronide, 3-(3ʹ-hydroxyphenyl) propanoic acid and hippuric acids, which appeared later (Tmax >6 h). The 24 h urinary recovery averaged 8.9% (molar basis) of the (poly)phenols consumed. Hepatic beta-oxidation of 3ʹ,4ʹ-dihydroxycinnamic acid and methylated conjugates occurred, but was limited (<0.04%). 3ʹ-Methylation exceeded 4ʹ-methylation and interindividual variability was high, especially for gut microbial metabolites (up to 168-fold).
M3 - Article
SN - 0308-8146
JO - FOOD CHEMISTRY
JF - FOOD CHEMISTRY
ER -