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In Vivo Targets of Pasteurella Multocida Toxin

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In Vivo Targets of Pasteurella Multocida Toxin. / Banu, Arshiya; Lax, Alistair J.; Grigoriadis, Agamemnon E.

In: International Journal of Molecular Sciences, Vol. 21, No. 8, 2739, 15.04.2020, p. 2739-2750.

Research output: Contribution to journalArticlepeer-review

Harvard

Banu, A, Lax, AJ & Grigoriadis, AE 2020, 'In Vivo Targets of Pasteurella Multocida Toxin', International Journal of Molecular Sciences, vol. 21, no. 8, 2739, pp. 2739-2750. https://doi.org/10.3390/ijms21082739

APA

Banu, A., Lax, A. J., & Grigoriadis, A. E. (2020). In Vivo Targets of Pasteurella Multocida Toxin. International Journal of Molecular Sciences, 21(8), 2739-2750. [2739]. https://doi.org/10.3390/ijms21082739

Vancouver

Banu A, Lax AJ, Grigoriadis AE. In Vivo Targets of Pasteurella Multocida Toxin. International Journal of Molecular Sciences. 2020 Apr 15;21(8):2739-2750. 2739. https://doi.org/10.3390/ijms21082739

Author

Banu, Arshiya ; Lax, Alistair J. ; Grigoriadis, Agamemnon E. / In Vivo Targets of Pasteurella Multocida Toxin. In: International Journal of Molecular Sciences. 2020 ; Vol. 21, No. 8. pp. 2739-2750.

Bibtex Download

@article{de95ba07155c4f239967c711bff2b5d7,
title = "In Vivo Targets of Pasteurella Multocida Toxin",
abstract = "Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins—G q/11, G 12/13, and G i/o —leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active β-catenin in a tissue-and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo. ",
keywords = "Pasteurella multocida toxin; G-proteins; proliferation; QE antibody; Ki67; pHH3; β-catenin, β-catenin, Pasteurella multocida toxin, PHH3, Proliferation, Ki67, G-proteins, QE antibody",
author = "Arshiya Banu and Lax, {Alistair J.} and Grigoriadis, {Agamemnon E.}",
year = "2020",
month = apr,
day = "15",
doi = "10.3390/ijms21082739",
language = "English",
volume = "21",
pages = "2739--2750",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "8",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - In Vivo Targets of Pasteurella Multocida Toxin

AU - Banu, Arshiya

AU - Lax, Alistair J.

AU - Grigoriadis, Agamemnon E.

PY - 2020/4/15

Y1 - 2020/4/15

N2 - Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins—G q/11, G 12/13, and G i/o —leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active β-catenin in a tissue-and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo.

AB - Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins—G q/11, G 12/13, and G i/o —leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active β-catenin in a tissue-and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo.

KW - Pasteurella multocida toxin; G-proteins; proliferation; QE antibody; Ki67; pHH3; β-catenin

KW - β-catenin

KW - Pasteurella multocida toxin

KW - PHH3

KW - Proliferation

KW - Ki67

KW - G-proteins

KW - QE antibody

UR - http://www.scopus.com/inward/record.url?scp=85083468997&partnerID=8YFLogxK

U2 - 10.3390/ijms21082739

DO - 10.3390/ijms21082739

M3 - Article

VL - 21

SP - 2739

EP - 2750

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 8

M1 - 2739

ER -

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