TY - JOUR
T1 - In vivo therapeutic potential of mesenchymal stromal cells depends on the source and the isolation procedure
AU - Bortolotti, Francesca
AU - Ukovich, Laura
AU - Razban, Vahid
AU - Martinelli, Valentina
AU - Ruozi, Giulia
AU - Pelos, Barbara
AU - Dore, Franca
AU - Giacca, Mauro
AU - Zacchigna, Serena
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Over the last several years, mesenchymal stromal cells (MSCs) have been isolated from different tissues following a variety of different procedures. Here, we comparatively assess the ex vivo and in vivo properties of MSCs isolated from either adipose tissue or bone marrow by different purification protocols. After MSC transplantation into a mouse model of hindlimb ischemia, clinical and histological analysis revealed that bone marrow MSCs purified on adhesive substrates exerted the best therapeutic activity, preserving tissue viability and promoting formation of new arterioles without directly transdifferentiating into vascular cells. In keeping with these observations, these cells abundantly expressed cytokines involved in vessel maturation and cell retention. These findings indicate that the choice of MSC source and purification protocol is critical in determining the therapeutic potential of these cells and warrant the standardization of an optimal MSC isolation procedure in order to select the best conditions to move forward to more effective clinical experimentation.
AB - Over the last several years, mesenchymal stromal cells (MSCs) have been isolated from different tissues following a variety of different procedures. Here, we comparatively assess the ex vivo and in vivo properties of MSCs isolated from either adipose tissue or bone marrow by different purification protocols. After MSC transplantation into a mouse model of hindlimb ischemia, clinical and histological analysis revealed that bone marrow MSCs purified on adhesive substrates exerted the best therapeutic activity, preserving tissue viability and promoting formation of new arterioles without directly transdifferentiating into vascular cells. In keeping with these observations, these cells abundantly expressed cytokines involved in vessel maturation and cell retention. These findings indicate that the choice of MSC source and purification protocol is critical in determining the therapeutic potential of these cells and warrant the standardization of an optimal MSC isolation procedure in order to select the best conditions to move forward to more effective clinical experimentation.
UR - http://www.scopus.com/inward/record.url?scp=84924599803&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2015.01.001
DO - 10.1016/j.stemcr.2015.01.001
M3 - Article
C2 - 25660405
AN - SCOPUS:84924599803
SN - 2213-6711
VL - 4
SP - 332
EP - 339
JO - Stem cell reports
JF - Stem cell reports
IS - 3
ER -